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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,764 results for: nts

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Showing: 861 - 880 of 18764 results

  3. pYO391

    Plasmid
    #235751
    Purpose
    Protein expression of ScVPS38, untagged + ScVPS34, untagged + FL ScVPS15-ZZ + ZZ-FL ScVPS30
    Depositor
    Roger Williams
    Article
    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d
    Inserts
    VPS34 (VPS34 Budding Yeast)
    VPS15 (VPS15 Budding Yeast)
    VPS38 (VPS38 Budding Yeast)
    VPS30 (VPS30 Budding Yeast)
    Tags
    3xTEV-ZZ and ZZ-3xTEV
    Expression
    Yeast
    Mutation
    S2A and T134A, I851R
    Promoter
    GALGAPDH
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    235751-plasmid-map-sequence-id-490579

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  4. pcDNA5/FRT/MALAT1[C8351G] (pAVA3169)

    Plasmid
    #239346
    Purpose
    Expresses full-length, driven by its own promoter MALAT1 with stability-compromised ENE(C8351G) and an 11-nt insertion (cgctcgacgta).
    Depositor
    Andrei Alexandrov
    Article
    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:
    Insert
    10,843 bp of MALAT1 of genomic locus amplified from genomic DNA of Hela cells (MALAT1 Human)
    Expression
    Mammalian
    Mutation
    An 11-nt insertion (cgctcgacgta) and stability-co…
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239346-plasmid-map-sequence-id-479130

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  10. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65773-plasmid-map-sequence-id-112657

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  12. pEXP5-pOsmB::mCherry

    Plasmid
    #53313
    Purpose
    mCherry reporter plasmid for OsmB promoter
    Depositor
    William DeGrado
    Insert
    OsmB (osmB E.coli)
    Tags
    mCherry
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pEXP5-pSoxS::mCherry

    Plasmid
    #53311
    Purpose
    mCherry reporter plasmid for SoxS promoter
    Depositor
    William DeGrado
    Insert
    SoxS promoter (soxS E.coli)
    Tags
    mCherry
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pUPD2 nickase D10A Cas9 (GB1690)

    Plasmid
    #160587
    Purpose
    Nickase D10A Cas9 for Nt and Ct fusion.
    Depositor
    Diego Orzaez
    Insert
    nickase D10A Cas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160587-plasmid-map-sequence-id-322079

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  16. pDIV069

    Plasmid
    #247966
    Purpose
    CRISPR-Cas9 vector containing a 20 nt protospacer sequence to target the IS1 locus where the DIVERSIFY gene landing platform is integrated in Aspergillus nidulans. pyrG marker
    Depositor
    Uffe Mortensen
    Insert
    Cas9-AMA1-IS1-gRNA-pyrG-ori-AmpR
    Use
    CRISPR
    Mutation
    none
    Available Since
    March 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    247966-plasmid-map-sequence-id-503027

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  17. pUPD2 nickase D10A Cas9 (GB1691)

    Plasmid
    #160588
    Purpose
    Nickase D10A Cas9 for Nt fusion.
    Depositor
    Diego Orzaez
    Insert
    nickase D10A Cas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160588-plasmid-map-sequence-id-322078

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  18. pColdII-SIRPT1-Ceratotherium-simum-simum

    Plasmid
    #78111
    Purpose
    Nt His-tag Ceratotherium-simum-simum Sacsin 89-1366
    Depositor
    Kalle Gehring
    Insert
    Sacsin Ceratotherium-simum-simum 89-1366
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78111-plasmid-map-sequence-id-145661

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  19. pColdII-SIRPT1-Mustela putorius furo

    Plasmid
    #78116
    Purpose
    Nt His-tag Mustela putorius furo Sacsin 94-1381
    Depositor
    Kalle Gehring
    Insert
    Sacsin Mustela putorius furo 94-1381
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78116-plasmid-map-sequence-id-145629

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Showing: 861 - 880 of 18764 results