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pcDNA-Wnt3A

Plasmid

#35908

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4

Plasmid

#27002

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pET22b-His6-UBCH7

Plasmid

#212711

Purpose

Bacterial expression of His6-UBCH7

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

UBCH7

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag003-MBP

Plasmid

#133450

Purpose

Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to N-terminus of MBP via a GSGE…

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee

Plasmid

#140836

Purpose

mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP_TRPM8

Plasmid

#64879

Purpose

Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

Depositor

Sebastián Brauchi

Article

Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

Insert

Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)

Tags

eGFP

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt7A

Plasmid

#35914

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7A (WNT7A Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt7B

Plasmid

#35915

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pHEN2-SG4 R105A

Plasmid

#196072

Purpose

Express mutant (R105A) SG4 in E.coli

Depositor

Shankar Balasubramanian

Article

Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

Insert

SG4 R105A

Tags

3XFLAG and 6XHis

Expression

Bacterial

Mutation

R105A mutation in SG4

Promoter

T7

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-Bst-LF

Plasmid

#145799

Purpose

Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

Depositor

Andrew Ellington

Article

Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

Insert

Bst-LF

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGP-CMV-GCaMP6s-CAAX

Plasmid

#52228

Purpose

Plasma membrane targeted GCaMP6s, calcium sensor

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

GCaMP6s-CAAX

Tags

6xHis and CAAX

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_EGFP

Plasmid

#183117

Purpose

Expresses flag-tagged EGFP in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

EGFP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4 DD

Plasmid

#26999

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Tags

HA

Expression

Mammalian

Mutation

C867A

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pEvol-NnSULT1C1-cysDNCQ

Plasmid

#188983

Purpose

biosynthesize sulfotyrosine in E. coli.

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Inserts

NnSULT1C1
cysD
cysN
cysC
cysQ

Expression

Bacterial

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP_mNG2(11)_Clathrin light chain

Plasmid

#82608

Purpose

Expresses mNG2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNG2(11)_Clathrin light chain

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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NW50

Plasmid

#133445

Purpose

Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW50

Expression

Bacterial

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM55261

Plasmid

#153210

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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NW30

Plasmid

#133444

Purpose

Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW30

Expression

Bacterial

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_H2B

Plasmid

#82605

Purpose

Expresses sfCherry2(11) tagged H2B in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_H2B

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Rac1

Plasmid

#180581

Purpose

ddGFP sensor of Rac1

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Rac1

Use

AAV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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