We narrowed to 17,732 results for: SHE

1. miRFP718nano-based inflammation reporter

   Plasmid

   #195753

   Purpose

   Reporter of inflamation

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   NF-kB-RE-miRFP718nano
   

   Use

   Tags

   miRFP718nano

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-TREtight-mTagBFP2-N2cG

   Plasmid

   #192838

   Purpose

   plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Depositor

   Ian Wickersham

   Insert

   mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Ubc.IkBSR-Flag Pgk.Cre

   Plasmid

   #22503

   Depositor

   Tyler Jacks

   Article

   Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

   Insert

   IkBSR
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p5'Entry-lck-5.5kb

   Plasmid

   #58890

   Purpose

   provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes

   Depositor

   Jeffrey Yoder

   Insert

   zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
   

   Use

   Gateway 5' entry clone

   Tags

   Expression

   Mutation

   mutated single nucleotide (T-->C, 13th nucleot…

   Promoter

   lymphocyte-specific protein tyrosine kinase (lck)…

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRF TpC hTERT intron-1

   Plasmid

   #171070

   Purpose

   Firefly-Renilla luciferase assay for hTERT promoter activity of hTERT intron-1

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   hTERT intron-1
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

   Plasmid

   #218710

   Purpose

   AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTRE-T2-IRES-His6-Ubiquitin

   Plasmid

   #107107

   Purpose

   Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.

   Depositor

   Michael E. Wright

   Insert

   human ubiquitin UBB (UBB Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Tet

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

   Plasmid

   #159912

   Purpose

   Mutagenesis of Kcnd3

   Depositor

   Larry Zweifel

   Insert

   Kcnd3 (Kcnd3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHPyV7-713a

   Plasmid

   #24728

   Depositor

   Christopher Buck

   Article

   Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

   Insert

   Full genome of Human polyomavirus 7
   

   Use

   Viral clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TC1405

    Plasmid

    #164877

    Purpose

    BFP-preSTOP-GFP reporter

    Depositor

    Tian Chi

    Article

    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

    Insert

    BFP-preSTOP-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. psd44-G13QL

    Plasmid

    #46828

    Purpose

    expression of mutant G protein alpha 13 in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    GNA13 (GNA13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Q226L (The mutation reduces GTPase activity resul…

    Promoter

    PGK promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-UbC-eGFP-F

    Plasmid

    #71545

    Purpose

    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

    Depositor

    Pantelis Tsoulfas

    Insert

    eGFP-F
    

    Use

    AAV

    Tags

    contains 20- amino-acid farnesylation signal from…

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EGFP-Miro1v4

    Plasmid

    #174111

    Purpose

    Expresses the peroxisomal variant (splice variant 4) of Miro with an N-terminal EGFP.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Miro1 (var4, peroxisomal splice variant)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG426-GAL-Alpha-synuclein-H50Q

    Plasmid

    #181724

    Purpose

    Galactose inducible expression of Alpha-synuclein-H50Q

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H50Q

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Sox17 GFP

    Plasmid

    #23334

    Depositor

    Sean Morrison

    Article

    Kim et al Cell. 2007 Aug 10. 130(3):470-83.

    Insert

    Sex determining region Y-box containing gene 17 (Sox17 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BiP-mGFP P495L

    Plasmid

    #62232

    Purpose

    Mutant of hamster BiP-mGFP unable to bind clients.

    Depositor

    Erik Snapp

    Article

    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

    Insert

    hamster BiP-mGFP-KDEL
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFBNb-cALFA

    Plasmid

    #159990

    Purpose

    For the protein expression with ALFA tag at C-terminus in insect cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHPyV6-607a

    Plasmid

    #24727

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    Full genome of Human polyomavirus 6
    

    Use

    Viral clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTW195b63

    Plasmid

    #136931

    Purpose

    Directed evolution of SpCas11

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII(N)-npuN
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CD44Z-c011

    Plasmid

    #175455

    Purpose

    Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge