We narrowed to 9,775 results for: Tec

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pYea5.1_C99_sfGFP

Plasmid

#207802

Purpose

Yeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-C100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch3_sfGFP

Plasmid

#207864

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch3_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 D122A pcDNA3

Plasmid

#206068

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA EIVK (E1658K) pcDNA3

Plasmid

#206066

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

E1658K

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-HiFi SpCas9-NLS-6xHis

Plasmid

#207378

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PET-Blackjack SpCas9-NLS-6xHis

Plasmid

#207375

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Blackjack SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 1005-1013 replaced with two glycine

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP12-talinC11-ABS3mut-mEmerald

Plasmid

#202383

Purpose

FKBP12-Talin(1974-2541)(K2443D)(V2444D)(K2445D)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

FKBP12-Talin(1974-2541)(K2443D)(V2444D)(K2445D)-mEmerald

Expression

Mammalian

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[K5A]-FLAG

Plasmid

#199326

Purpose

mutated ubiquitination site (ubiquitination inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 5 to Alanine

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[K5R]-FLAG

Plasmid

#199325

Purpose

mutated ubiquitination site (ubiquitination active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 5 to Arginine

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199A]-FLAG

Plasmid

#199313

Purpose

mutated phosphorylation site (kinase acitivity inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Alanine

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_CI-hCOL4A5-Nluc_R1683X

Plasmid

#198111

Purpose

lentiviral expression of hCOL4A4_R1683Xin mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

R1683X

Promoter

EF1a

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_CI-hCOL4A5-Nluc_C1684X

Plasmid

#198112

Purpose

lentiviral expression of hCOL4A4_C1684X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

C1684X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_CI-hCOL4A5-Nluc_C29X

Plasmid

#198107

Purpose

lentiviral expression of hCOL4A5_C29X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

C29X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_CI-hCOL4A5-Nluc_R1563X

Plasmid

#198109

Purpose

lentiviral expression of hCOL4A4_R1563Xin mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

R1563X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_CI-hCOL4A5-Nluc_S36X

Plasmid

#198108

Purpose

lentiviral expression of hCOL4A4_S36X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

S36X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_CI-hCOL4A5-Nluc

Plasmid

#198106

Purpose

lentiviral expression of hCOL4A4 in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Promoter

EF1a

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmCUP_325-376-R329E-GB1_AA

Plasmid

#148263

Purpose

Bacterial Expression of DmCup_325-376-R329E

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmCup_325-376-R329E (cup Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmCUP_325-376-R329ER336E-GB1_AA

Plasmid

#148264

Purpose

Bacterial Expression of DmCup_325-376-R329ER336E

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmCup_325-376-R329ER336E (cup Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmCUP_325-376-R329ER336ER375E-GB1_AA

Plasmid

#148265

Purpose

Bacterial Expression of DmCup_325-376-R329ER336ER375E

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmCup_325-376-R329ER336ER375E (cup Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmCUP_325-376-R336E-GB1_AA

Plasmid

#148266

Purpose

Bacterial Expression of DmCup_325-376-R336E

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmCup_325-376-R336E (cup Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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