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pLenti-gRNA7-3

Plasmid

#248531

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA7-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA8-2

Plasmid

#248532

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA8-2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1623

Plasmid

#239833

Purpose

WT OrufIscB E. coli expression for recombinant purification

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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mt TEFM

Plasmid

#205058

Purpose

TEFM protein expression

Depositor

Smita Patel

Article

Sultana et al J Biol Chem. 2017 Nov 3;292(44):18145-18

Insert

TEFM (TEFM Human)

Expression

Bacterial

Mutation

Codon optimized

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pXTm-zebrafish Tspan4a

Plasmid

#210327

Purpose

Expresses Tetraspanin-4a (TSPAN4a) as migrasome marker

Depositor

Li Yu

Article

Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

Insert

Tetraspanin 4a (tspan4a Zebrafish)

Use

Fish expression

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAX-2

Plasmid

#210130

Purpose

knock out BAX in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Apoptosis regulator BAX (BAX Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAX-1

Plasmid

#210013

Purpose

knock out BAX in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Apoptosis regulator BAX (BAX Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-MTdTc302

Plasmid

#207458

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTc302 (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Mutation

Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ273: pRS426-His-MBP-GtFz1_PSP1

Plasmid

#205258

Purpose

S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinCS

Plasmid

#200309

Purpose

Lentivirus vector expressing vinculin with FRET conformational sensor

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

VinculinCS

Use

Lentiviral

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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DCIP

Plasmid

#193860

Purpose

Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

NLS-mCherry-GFP1-10

Tags

SV40 NLS

Expression

Plant

Promoter

35S

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCA27-pHR-MiniCMV-TriTag (mTagBFP)

Plasmid

#199458

Purpose

Expression of TriTag (mTagBFP) in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TriTag

Use

Lentiviral

Expression

Mammalian

Promoter

MiniCMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70DA9-Pct5-DadhABC(A2)

Plasmid

#191632

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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TaRGET-ABE-C3.1 ver4.0

Plasmid

#188250

Purpose

Hypercompact adenine base editors

Depositor

Yong-Sam Kim

Article

Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

Insert

TaRGET-ABE3.1

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEA183

Plasmid

#187873

Purpose

Expresses eGFP under the virB promoter.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

eGFP

Expression

Bacterial

Promoter

Agrobacterium virB promoter

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_NWS.HA.V5_mCherry-NLS

Plasmid

#178283

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

NWS.HA.V5 and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.NWS.Ollas_mCherry-NLS

Plasmid

#178263

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.NWS.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_C.HA.V5_mCherry-NLS

Plasmid

#178242

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

C.HA.V5 and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15001-AncBE4max

Plasmid

#163016

Purpose

plasmid expressing AncBE4max

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

AncBE4max

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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SNX10-PX (1-201)

Plasmid

#119091

Purpose

Bacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX10-PX (1-201) (SNX10 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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