We narrowed to 166,303 results for: addgene

1. PHKG2_HUMAN_D0

   Plasmid

   #79736

   Purpose

   This plasmid encodes the kinase domain of PHKG2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   PHKG2 (PHKG2 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. VRK2_HUMAN_D0

   Plasmid

   #79737

   Purpose

   This plasmid encodes the kinase domain of VRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   VRK2 (VRK2 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. ADCK4 gRNA (BRDN0001145019)

   Plasmid

   #76560

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ADCK4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ADCK4
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC_N_CDK7

   Plasmid

   #111684

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   CDK7 (CDK7 Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX4

   Plasmid

   #183903

   Purpose

   donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   GFP
   

   Expression

   Insect

   Promoter

   Aedes aegypti PUb

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX3

   Plasmid

   #183904

   Purpose

   donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   GFP
   

   Expression

   Insect

   Promoter

   Aedes aegypti Polyubiquitin

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PAX6 sgRNA2

   Plasmid

   #68465

   Purpose

   targeting PAX6 gene

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   sgRNA targeting PAX6 (PAX6 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.2 mir-1-1 reporter hsa-mir-128-1

   Plasmid

   #46675

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-128-1 (MIR128-1 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. OVA-miSFIT-2xPerfect

   Plasmid

   #124673

   Purpose

   Tuning expression of cytosolic Ovalbumin coupled to EGFP

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   Cyto-Ovalbumin
   

   Expression

   Mammalian

   Mutation

   Truncated OVA for cytoplasmic expression

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-cGAS-N-HA (murine)

    Plasmid

    #130916

    Purpose

    Expresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    murine cGAS N terminus
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    murine cGAS N terminus (aa 1-147)

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

    Plasmid

    #193915

    Purpose

    Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. gh178

    Plasmid

    #106828

    Purpose

    expression of gRNA targeting GYS1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    GYS1 (GYS1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBC2T-Ypet-FtnA

    Plasmid

    #108969

    Purpose

    expresses BC2-tag-Ypet-FtnA in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    FtnA (ftnA Escherichia coli)
    

    Tags

    BC2-tag and Ypet

    Expression

    Mammalian

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. sTR060

    Plasmid

    #177370

    Purpose

    Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

    Depositor

    Jerome Bonnet, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    Trigger RNA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMKO shRNA Bmf

    Plasmid

    #17236

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bmf shRNA (BMF Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

16. 8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124541

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
    

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-NCre-MBP6-WPRE

    Plasmid

    #193916

    Purpose

    Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    NCre-MBP6
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE bleo mouse IRS-2

    Plasmid

    #11447

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

    Plasmid

    #133788

    Purpose

    CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized St3Cas9
    

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.2 mir-1-1 reporter mir16-1 wt

    Plasmid

    #46683

    Purpose

    Expresses wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1 wt (MIR16-1 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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