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p223-Pulse-Switch

Plasmid

#217889

Purpose

Lentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-ARMS1-ProLink2-MRGPRX3

Plasmid

#213962

Purpose

Expresses MRGPRX3 in mammalian cells

Depositor

Christa Müller

Article

Al Hamwi et al Commun Biol. 2024 Jan 6;7(1):52. doi: 10

Insert

MRGPRX3 (MRGPRX3 )

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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AA018

Plasmid

#215976

Purpose

Fragmid fragment: (N' terminus) 4xVP16 transcritional activators

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; VP64_v1.1

Use

Fragment

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_162

Plasmid

#216092

Purpose

Cas9 [Sp] CRISPRi targeting CD47, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD47 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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AA134

Plasmid

#216044

Purpose

Fragmid fragment: (C' terminus) SV40NLS; Linker; MeCP2

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

SV40NLS_v1.7; Linker_v4.1; MeCP2_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA006

Plasmid

#216056

Purpose

Fragmid fragment: (2A Selection) Blasticidin resistance

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

T2A_v1.1; BlastR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA199

Plasmid

#216050

Purpose

Fragmid fragment: (C' terminus) p65; NpNLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

p65_v1.1; NpNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA266

Plasmid

#216064

Purpose

Fragmid fragment: (2A Selection) TagRFP

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; TagRFP_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA267

Plasmid

#216065

Purpose

Fragmid fragment: (2A Selection) TagRFP657

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; TagRFP657_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA200

Plasmid

#216051

Purpose

Fragmid fragment: (C' terminus) p65; HSF1; NpNLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

p65_v1.2; HSF1_v1.1; NpNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA414

Plasmid

#215991

Purpose

Fragmid fragment: (N' terminus) conditional degradation

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; V5_v1.1; HaloTag_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA093

Plasmid

#216042

Purpose

Fragmid fragment: (C' terminus) ZIM3 KRAB

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NpNLS_v1.1; KRAB_v3.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA175

Plasmid

#216062

Purpose

Fragmid fragment: (2A Selection) EGFP

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; EGFP_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA368

Plasmid

#215972

Purpose

Fragmid fragment: (Pol 2 promoter) ubiquitous expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Act5C_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA367

Plasmid

#215971

Purpose

Fragmid fragment: (Pol 2 promoter) Copper-induced expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

MT_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA413

Plasmid

#215990

Purpose

Fragmid fragment: (N' terminus) conditional degradation

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; V5_v1.1; dTAG_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA226

Plasmid

#216027

Purpose

Fragmid fragment: (Cas protein) Cas7-11

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas7-11_v1.1 [Di]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA276

Plasmid

#216066

Purpose

Fragmid fragment: (2A Selection) Neomycin resistance

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; NeoR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA043

Plasmid

#215964

Purpose

Fragmid fragment: (Pol 2 promoter) human Phosphoglycerate Kinase promoter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

PGK_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA279

Plasmid

#216001

Purpose

Fragmid fragment: (Selection) fluorescent reporter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; TagRFP_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge