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pAAV-Ef1a-FAS-EGFP-WPRE-pA

Plasmid

#37091

Purpose

Expresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

Depositor

Bernardo Sabatini

Publication

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

d1eGFP

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EGFP-Ahi1

Plasmid

#30494

Depositor

Joseph Gleeson

Publication

Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

Insert

Ahi1 (AHI1 Human)

Tags

EGFP

Expression

Mammalian

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pQCXIH-Flag Smad3 WT

Plasmid

#27025

Depositor

Joan Massague

Publication

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
MSCV FLIPi P2G_Thy1.1 PTEN

Plasmid

#19749

Depositor

Richard Hynes

Publication

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pAd-CCN1

Plasmid

#12519

Depositor

Tong-Chuan He

Publication

Si et al Mol Cell Biol. 2006 Apr . 26(8):2955-64.

Insert

CCN1 (Ccn1 Mouse)

Expression

Mammalian

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFastBac1 hBRM

Plasmid

#1956

Depositor

Robert Kingston

Publication

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BRM (SMARCA2 Human)

Tags

flag

Expression

Insect

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
HA-FOXO3a TM delta CT

Plasmid

#1797

Depositor

Michael Greenberg

Publication

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

T32A, S253A, S315A, delta CT (aa 468-673)

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSB-CMV-MCS-Puro cPLA2-EGFP

Plasmid

#162568

Purpose

Used as a measure of cPLA2 activation in live cells with an EGFP tag.

Depositor

Philipp Niethammer

Publication

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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PCS4 3XFLAG-PPARgamma1

Plasmid

#78769

Purpose

To overexpress PPARgamma1 in Mammalian Cells

Depositor

Jaewhan Song

Publication

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_1 (Pparg Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p403-BrdU-Inc

Plasmid

#71789

Purpose

BrdU-incorporating plasmid with selectable marker HIS3

Depositor

Oscar Aparicio

Publication

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PTEF1-yEGFPCLN2PEST-pRS406

Plasmid

#64406

Purpose

TEF promoter driven GFP expression

Depositor

Claudia Vickers

Publication

Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

Insert

TEF1 promoter (TEF1 Budding Yeast)

Tags

yEGFPCLN2PEST (destabilized yeast EGFP)

Expression

Yeast

Promoter

TEF1 promoter

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-C3-Sec6

Plasmid

#53757

Purpose

pEGFP-C3 plasmid that expresses Sec6

Depositor

Channing Der

Publication

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec6 (EXOC3 Human)

Tags

GFP

Expression

Mammalian

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBS2E

Plasmid

#55169

Purpose

Empty vector, integration at lacA, ampr, mlsr

Depositor

Thorsten Mascher

Publication

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTBNde(min)

Plasmid

#15125

Depositor

Franco Pagani

Publication

Pagani et al Hum Mol Genet. 2003 May 15. 12(10):1111-

Insert

alpha-globin EDB fibronectin minigene (FN1 Human)

Expression

Mammalian

Promoter

alpha-globin

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRK Smad3 deltaC Flag

Plasmid

#12626

Depositor

Rik Derynck

Publication

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

Smad3 (SMAD3 Human)

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated Smad3 (aa 1-382)

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
GEV 1

Plasmid

#12615

Depositor

John Louis

Publication

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with Bcl2 as insert (Bcl2 Mouse)

Tags

G protein and His

Expression

Bacterial

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

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MGAT2-RpHLuorin2

Plasmid

#171718

Purpose

Cis-/medial-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Publication

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS1099: Dual-sgRNA.Design 4

Plasmid

#159537

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Publication

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 with two guide RNA cassettes

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-PLAA

Plasmid

#85669

Purpose

Expression of PLAA with N-terminal GFP tag

Depositor

Hemmo Meyer

Publication

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

PLAA (PLAA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

Plasmid

#85717

Purpose

Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

Depositor

Erik Sontheimer

Publication

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Sp dCas9
telomere sgRNA

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO and U6

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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