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JAK1 gRNA (BRDN0001149081)

Plasmid

#76394

Purpose

3rd generation lentiviral gRNA plasmid targeting human JAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

JAK1 (JAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RPS6KA3 gRNA (BRDN0001147852)

Plasmid

#75944

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA3 (RPS6KA3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-31-PGK-GFP-Blast

Plasmid

#31515

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
PDGFRA gRNA (BRDN0001148373)

Plasmid

#75493

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDGFRA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDGFRA (PDGFRA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ERN1 gRNA (BRDN0001149471)

Plasmid

#76410

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERN1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERN1 (ERN1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK14 gRNA (BRDN0001145809)

Plasmid

#77923

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK14

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK14 (MAPK14 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK13 gRNA (BRDN0001147672)

Plasmid

#76625

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK13

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK13 (CDK13 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALDH18A1 gRNA (BRDN0001149056)

Plasmid

#77994

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALDH18A1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALDH18A1 (ALDH18A1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TGMP.shKras.1422

Plasmid

#59913

Purpose

Tet on shKras for conditional Kras knockdown

Depositor

Tyler Jacks

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

Kras (Kras Mouse)

Use

Retroviral

Available Since

Dec. 16, 2014

Availability

Academic Institutions and Nonprofits only

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KIF1C

Plasmid

#155687

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

KIF1C (KIF1C Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3

Plasmid

#106206

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A + mRuby3 fusion

Promoter

CAG

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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ULK1 gRNA (BRDN0001145850)

Plasmid

#75737

Purpose

3rd generation lentiviral gRNA plasmid targeting human ULK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ULK1 (ULK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ETV1

Plasmid

#98623

Purpose

Gateway entry TOPO TA cloning vector for ETV1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ETV1 (Etv1 Mouse)

Use

Topo ta cloning vector

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap-FLEX.SF-iGluSnFR.S72A

Plasmid

#106182

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

hSynapsin

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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ERN1 gRNA (BRDN0001146341)

Plasmid

#76408

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERN1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERN1 (ERN1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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JAK1 gRNA (BRDN0001144778)

Plasmid

#76393

Purpose

3rd generation lentiviral gRNA plasmid targeting human JAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

JAK1 (JAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.5 shETV4-B

Plasmid

#74976

Purpose

shETV4 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV4 (ETV4 Human)

Use

RNAi

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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MAPK14 gRNA (BRDN0001148417)

Plasmid

#77924

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK14

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK14 (MAPK14 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ERN1 gRNA (BRDN0001162304)

Plasmid

#76407

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERN1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERN1 (ERN1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B GG

Plasmid

#89451

Purpose

Expression of GFP-tagged human Rab27B GG in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B GG (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

GG (geranyl geranyl site is mutated)

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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