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qTAG-N-Blast-mStayGold

Plasmid

#227251

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mStayGold cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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P4-TadCBEd

Plasmid

#225143

Purpose

Packaging plasmid for TadCBEd in v3b PE-eVLP architecture

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-TadCBEd

Expression

Mammalian

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xFLAG-Puro

Plasmid

#207728

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-mScarlet-Puro

Plasmid

#207722

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-dTAG

Plasmid

#207705

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-mScarlet-Blast

Plasmid

#207711

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Puro-mNeon

Plasmid

#207687

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-mCherry

Plasmid

#199247

Purpose

Fluorescent labelling of bacterial cells with mCherry

Depositor

Uwe Mamat

Article

Mamat et al Appl Environ Microbiol. 2023 May 17:e003

Insert

Codon-optimized mCherry gene

Expression

Bacterial

Mutation

Codon optimization for Xanthomonadaceae

Promoter

Pc promoter from class III integron of Delftia a…

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-E2-Crimson

Plasmid

#111252

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with E2-Crimson

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

E2-Crimson

Expression

Bacterial

Promoter

Pc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG2b_M18

Plasmid

#105931

Purpose

Expression plasmid coding for heavy chain of mouse IgG2b antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG2b (Ighg2b Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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R619-X67-527: His6-SARS-CoV-2 N

Plasmid

#170204

Purpose

E. coli expression of SARS-CoV-2 nucleocapsid protein

Depositor

Dominic Esposito

Insert

SARS-CoV-2 N (N SARS-CoV-2)

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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HO-Poly-HO

Plasmid

#51661

Purpose

yeast plasmid for integration of target sequence at HO locus

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ Channel [K89/34R]

Plasmid

#128628

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ Channel (Rat). Derived from hybridoma K89/34.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv2.1 K+ Channel (Rat) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Nav channel [K58/35R]

Plasmid

#128623

Purpose

Mammalian Expression Plasmid of anti-Pan-Nav channel. Derived from hybridoma K58/35.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Nav Channel Pan recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Kv1.2 K+ Channel [K14/16R]

Plasmid

#128614

Purpose

Mammalian Expression Plasmid of anti-Kv1.2 K+ channel (Human). Derived from hybridoma K14/16.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv1.2 K+ Channel (Human) recombinant mouse monoclonal antibody (KCNA2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(B)R2 [N81/37.1R]

Plasmid

#114544

Purpose

Mammalian Expression Plasmid of anti-GABA(B)R2 (Human). Derived from hybridoma N81/37.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(B)R2 (Homo sapiens) recombinant mouse monoclonal antibody (GABBR2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2-Hsp27-S78D

Plasmid

#85183

Purpose

Hsp27 expression plasmid

Depositor

Ugo Moens

Insert

Hsp27 (HSPB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S78D, phosphomimicking mutation

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2-Hsp27-S15D

Plasmid

#85182

Purpose

Hsp27 expression plasmid

Depositor

Ugo Moens

Insert

Hsp27 (HSPB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S15D, phosphomimicking mutation

Promoter

CMV

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBS ZfHoxb1b

Plasmid

#27520

Depositor

Victoria Prince

Insert

Hoxb1b (hoxb1b Zebrafish)

Use

Riboprobe synthesis

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only