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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 79,221 results for: rest

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Showing: 8921 - 8940 of 79221 results

  1. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

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  2. pCDNA3 Flag MKK3b(Glu)

    Plasmid
    #50449
    Purpose
    activates p38 in the signaling pathway
    Depositor
    Roger Davis
    Publication
    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MKK3b (MAP2K3 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    changed Serine 218 to Glutamic Acid, changed Thre…
    Promoter
    CMV
    Available since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  5. HA-CHMP4B

    Plasmid
    #139013
    Purpose
    Expresses HA-tagged human CHMP4B in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human CHMP4B
    Tags
    HA
    Expression
    Mammalian
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139013-plasmid-map-sequence-id-274403

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  9. pPD95_77

    Plasmid
    #1495
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, rf2204, gfpN, IVSR, gfpS65C, IVSS, IVST, gf…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1495-plasmid-map-sequence-id-106

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  15. Fireworks GFP[PTC-] (AVA2598)

    Plasmid
    #85445
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.
    Depositor
    Joan Steitz
    Publication
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Expression
    Mammalian
    Available since
    Feb. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    85445-plasmid-map-sequence-id-171491

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  19. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

    Plasmid
    #22931
    Purpose
    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)
    Depositor
    Alexandra Newton
    Publication
    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PHLPP1-deltaC (PHLPP1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    PHLPP-deltaC, deletion of PDZ binding domain--the…
    Promoter
    CMV
    Available since
    Feb. 4, 2010
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 8921 - 8940 of 79221 results