We narrowed to 286,989 results for: Gene

1. pYPQ141D-peg

   Plasmid

   #141081

   Purpose

   Gateway compatible prime editing gRNA empty backbone vector

   Depositor

   Yiping Qi

   Article

   Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. ChR2-2A-Halo

   Plasmid

   #22047

   Purpose

   Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

   Depositor

   Edward Boyden

   Article

   Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

   Insert

   ChR2-GFP-2A-Halo-YFP
   

   Tags

   GFP and YFP

   Expression

   Mammalian

   Mutation

   Note that there is a S147R mut in YFP. This mutat…

   Promoter

   CMV

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a/Cas9-Cys

   Plasmid

   #53261

   Purpose

   Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

   Depositor

   Hyongbum Kim

   Article

   Ramakrishna et al Genome Res. 2014 Apr 2.

   Insert

   Cas9-Cys
   

   Tags

   6X His tag, HA epitope, and NLS

   Expression

   Bacterial

   Mutation

   Additional Cysteine is added to the C-terminal re…

   Promoter

   T7 Promoter

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-DDX3X

   Plasmid

   #70648

   Purpose

   Expresses WT DDX3X in mammalian cells under blasticidin selection.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   DDX3X (DDX3X Human)
   

   Use

   Retroviral

   Tags

   3x FLAG

   Expression

   Mammalian

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pMGS7 (AAVS1sgRNA)

   Plasmid

   #126582

   Purpose

   A CRISPR/Cas9 construct to target the AAVS1 locus in humans

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   AAVS1 sgRNA
   

   Expression

   Mammalian

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVAX1-BMP2

   Plasmid

   #137909

   Purpose

   Constitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNA

   Depositor

   Georg Feichtinger

   Article

   Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

   Insert

   BMP2 (BMP2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBP-T7_RBS-His6-Thrombin

   Plasmid

   #72979

   Purpose

   T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7+RBS+His6+thrombin
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCT310

   Plasmid

   #111140

   Purpose

   Bacterial expression of 6His-dCas9-3XFlag

   Depositor

   Robert Tjian

   Article

   Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

   Insert

   dCas9
   

   Tags

   3X Flag and 6X His

   Expression

   Bacterial

   Mutation

   D10A - H840A

   Promoter

   T7

   Available Since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCVhyg-3xT7-CA-ChREBP

   Plasmid

   #171029

   Purpose

   Expresses 3xT7-tagged constitutive active form of ChREBP in mammalian cells

   Depositor

   Kai Ge

   Article

   Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

   Insert

   CA-ChREBP (Mlxipl Mouse)
   

   Use

   Retroviral

   Tags

   3xT7

   Expression

   Mammalian

   Mutation

   amino acids 240 - 864

   Promoter

   5' LTR

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-mTurquoise2

    Plasmid

    #99122

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mTurquoise2 from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mTurquoise2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-mRuby2

    Plasmid

    #99123

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mRuby2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD11b promoter in pGEM3zf(-)

    Plasmid

    #26168

    Depositor

    Daniel Tenen

    Article

    Dziennis et al Blood. 1995 Jan 15. 85(2):319-29.

    Insert

    CD11b promoter (ITGAM Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEB-Tg

    Plasmid

    #28220

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    SV40 large T
    

    Expression

    Mammalian

    Available Since

    April 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. single cut NH-donor

    Plasmid

    #83575

    Purpose

    eGFP reporter donor containing single SA cutting site for NHEJ knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Nhej donor

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBV65

    Plasmid

    #108363

    Purpose

    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

    Depositor

    Scott Moye-Rowley

    Article

    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

    Insert

    loxP-natMX6-CgMET3prom-cre-loxP
    

    Use

    Gene disruption marker for candida glabrata

    Promoter

    C. glabrata MET3

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3-Flag MKK6

    Plasmid

    #13517

    Depositor

    Roger Davis

    Article

    Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

    Insert

    MKK6 (MAP2K6 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-TRIM28deltaRING

    Plasmid

    #124952

    Purpose

    Mammalian expression of Human TRIM28 with deleted RING domain

    Depositor

    Michelle Barton

    Article

    Li et al Oncogene. 2017 May 25;36(21):2991-3001.

    Insert

    hTRIM28 (TRIM28 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of RING domain

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sg-A

    Plasmid

    #83807

    Purpose

    Express sgRNA targeting SA site

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEF-VSV-G

    Plasmid

    #41792

    Purpose

    VSV-G-expressing envelope plasmid

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Vesicular stomatitis virus G glycoprotein, Indiana strain
    

    Expression

    Mammalian

    Promoter

    CEF

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSR657

    Plasmid

    #65214

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-2 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    p10 and polyhedrin

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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