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TDG004_pLenti_CMVtight_Kir2.1_CFP

Plasmid

#178820

Purpose

tet-on expression of an inward-rectifier potassium channel Kir2.1 carrying an CFP tag

Depositor

Adam Cohen

Publication

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

Kir2.1_CFP (Kcnj2 Mouse)

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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RNF6_pLX307

Plasmid

#98366

Purpose

Lentiviral expression of RNF6

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

RNF6 (RNF6 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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LIMD1_pLX307

Plasmid

#98349

Purpose

Lentiviral expression of LIMD1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

LIMD1 (LIMD1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_NFKB1_WT

Plasmid

#82149

Purpose

Gateway Donor vector containing NFKB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFKB1 (NFKB1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-CDC2L6

Plasmid

#23429

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDC2L6 (CDK19 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PIK3CB

Plasmid

#23542

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3CB (PIK3CB Human)

Use

Gateway Cloning

Mutation

I6L

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DCAMKL2

Plasmid

#23604

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DCAMKL2 (DCLK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pIRES.Puro.EGFP

Plasmid

#24176

Depositor

Robert Sobol

Publication

Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Expression

Mammalian

Mutation

x

Available since

April 9, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pHR-scFv-GCN4-mStayGold-GB1-dWPRE

Plasmid

#231598

Purpose

Express scFv_GCN4-mStayGold for SunTag translation imaging

Depositor

Marvin Tanenbaum

Publication

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

scFv-GCN4-mStayGold

Use

Lentiviral

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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PM-B

Plasmid

#209876

Purpose

Dimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

Depositor

Sarah Cohen

Publication

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ddGFP B

Tags

Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3432

Plasmid

#144908

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Publication

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NR3C1 (NR3C1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDL026_HA-rb_IgG_LC_15F11

Plasmid

#193623

Purpose

HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

Depositor

Jennifer DeLuca

Publication

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-HA_15F11 rabbit light chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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BPK1520_puroR

Plasmid

#173901

Purpose

SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

Depositor

Ronald Cohn

Publication

Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV/U6

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR221_SLC4A7

Plasmid

#132204

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A7 (SLC4A7 Human)

Expression

Mammalian

Available since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pCggRO-reporter

Plasmid

#124582

Purpose

Reporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)

Depositor

Matthias Heinemann

Publication

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

CggR promoter element

Expression

Yeast

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-AKT3

Plasmid

#116711

Purpose

Lentiviral expression of AKT3

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT3 (AKT3 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_SOX2_P2A_Hygro_Barcode

Plasmid

#120480

Purpose

Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX2 (SOX2 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF4_P2A_Hygro_Barcode

Plasmid

#120455

Purpose

Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-24

Plasmid

#79939

Purpose

PH-Late1-I3-01-myc

Depositor

Neil King

Publication

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EPN-24

Expression

Bacterial and Mammalian

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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shBIRC5 # 2

Plasmid

#42554

Depositor

William Hahn

Publication

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shBIRC5 (BIRC5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only