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Plasmid#196921PurposeThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the Renilla coding sequence in the ΔIRE-TREAT reporterDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
BALF5-D755N-D757N
Plasmid#192455PurposeExpresses BALF5 with D755N and D757N mutationsDepositorInsertBALF5 (D755N&D757N) (BALF5 Epstein-Barr Virus)
UseTagsFLAGExpressionMammalianMutationD755N & D757NPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
IRE-miR21mutant-SunTag-TREAT
Plasmid#196933PurposeSunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE-let7-SunTag-TREAT
Plasmid#196926PurposeSunTag cassette was added in the coding region in the let7-IRE-TREAT reporterDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE-let7R-SunTag-TREAT
Plasmid#196927PurposeSunTag cassette was added in the coding region in the let7R-IRE-TREAT reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
B270 + SMARCAL1 sgSTOP
Plasmid#100717PurposeB270 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOPDepositorInsertsgSTOP targeting SMARCAL1 (cloned using BbsI) and ATP1A1 (SMARCAL1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promotersAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
B52 + SMARCAL1 sgSTOP
Plasmid#100715PurposeB52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorInsertsgSTOP targeting SMARCAL1 (cloned using BbsI)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promotersAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
B52 + SPRTN sgSTOP
Plasmid#100709PurposeB52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorInsertsgSTOP targeting SPRTN (cloned using BbsI)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promotersAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
B52 + TIMELESS sgSTOP
Plasmid#100713PurposeB52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorInsertsgSTOP targeting TIMELESS (cloned using BbsI)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promotersAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
B52 + PARP4 sgSTOP
Plasmid#100711PurposeB52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorInsertsgSTOP targeting PARP4 (cloned using BbsI)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promotersAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
B52 + PIK3R1 sgSTOP
Plasmid#100714PurposeB52 plasmid expressing PI3KR1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorInsertsgSTOP targeting PIK3R1 (cloned using BbsI)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promotersAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGTQL1221YC
Plasmid#61705PurposeGateway compatible BiFC vector (pEarleyGate202-YC)DepositorTypeEmpty backboneUseBifc assayTagsYFP-C and FLAGExpressionPlantMutationPromoter35SAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCM167.2-Venus-VF-hADAM10(aa1-213)
Plasmid#189554PurposeProtein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 1-213PromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE-SunTag
Plasmid#196920PurposeThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporterDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7-SunTag
Plasmid#196928PurposeLet7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7R-SunTag
Plasmid#196929PurposeReverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
miR21mutant-IRE-TREAT
Plasmid#196931PurposeMutated mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporterDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
miR21-IRE-TREAT
Plasmid#196930Purposemir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7-IRE-TREAT
Plasmid#196925PurposeLet7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
sm-9xGCN4-Renilla-MS2
Plasmid#178009PurposeExpresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in mammalian cellsDepositorInsertsm-9xGCN4
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only