We narrowed to 7,648 results for: Pol

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pUC19-TgKI-MCS-p2A-tdTomato-polyA

Plasmid

#174027

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-tdTomato

Use

Zebrafish knock-in tagging

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

Plasmid

#174025

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Mutation

Y40N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

Plasmid

#48294

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Expression

Insect

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)

Plasmid

#48308

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6

Expression

Bacterial

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-Phi29 (LM2708)

Plasmid

#208965

Purpose

Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

Phi29-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

Phi29(-exo;D169A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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PL452-hGH-polyA-PGK-neoR

Plasmid

#191681

Purpose

Donor plasmid backbone PGK-neoR

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Type

Empty backbone

Expression

Mammalian

Mutation

WT

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLE1 N-ISCmut

Plasmid

#160803

Purpose

Express N-terminal ISC mutant POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLE1 N-ISCmut (POLE Human)

Use

Retroviral

Tags

3xFLAG

Mutation

shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(WT)-IRES-Neo

Plasmid

#128663

Purpose

mammalian expression of Flag-Polß(WT) protein with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaCTD-HA.SV40(polyA)

Plasmid

#156163

Purpose

Expresses truncated (AA 1-954) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Truncated THBS1 (amino acid residues 1-954)

Promoter

CMV

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaTSR1-HA.SV40(polyA)

Plasmid

#156162

Purpose

Expresses murine Thrombospondin-1 (THBS1) with deletion (AA 383-548) and HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with deletion of amino acid residues 383-548

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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YIp211-P30-His-pol30(K164R)-3'

Plasmid

#99548

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K164R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Dnmt3b1-Poly(A)-NeoR

Plasmid

#65553

Purpose

Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

mCherry

Expression

Mammalian

Mutation

isoform 1

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

Plasmid

#213025

Purpose

Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

Depositor

Martin S. Taylor

Article

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

LINE-1 ORF2p (1-1275)

Tags

3C-3xFlag (ORF2p)

Expression

Insect

Promoter

Polyhedrin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

Plasmid

#48283

Depositor

Scott Gradia

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-2-SV40 polyA

Plasmid

#190866

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode2

Use

AAV

Mutation

WT

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMJO-6 (minus T7 RNA polymerase binding site)

Plasmid

#31991

Depositor

Mark Osborn

Article

Osborn et al Hum Gene Ther. 2011 Jun 13.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

Plasmid

#174029

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-mScarlet

Use

Zebrafish knock-in tagging

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense

Plasmid

#46836

Purpose

Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-polyubiquitin_6xHis

Plasmid

#256080

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Article

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Polyubiquitin

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pET15Sumo2-Polio3C_GTM_001

Plasmid

#240387

Purpose

Expresses Polio virus protease in E. coli

Depositor

Gaetano Montelione

Insert

3C protease

Tags

6xHis, SUMO

Expression

Bacterial

Promoter

T7 lac

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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