We narrowed to 1,046 results for: Psp;

1. EED-KO-gRNA-1

   Plasmid

   #131321

   Purpose

   gRNA to knockout EED. Use with EED-KO-gRNA-2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

2. pTRE/SP M87 N184X

   Plasmid

   #92373

   Purpose

   Inducible expression of M87 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-460 bp deleted, N184X

   Promoter

   Tet-responsive P tight

   Available Since

   June 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTRE/SP S245X with 5'UTR

   Plasmid

   #92376

   Purpose

   Inducible expression of S245X spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   S245X

   Promoter

   Tet-responsive P tight

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTRE/SP M87 S245X

   Plasmid

   #92374

   Purpose

   Inducible expression of M87 S245X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-460 bp deleted, S245X

   Promoter

   Tet-responsive P tight

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTRE/SP M1 S245X

   Plasmid

   #92372

   Purpose

   Inducible expression of M1S245X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, S245X

   Promoter

   Tet-responsive P tight

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTRE/SP M1 N184X

   Plasmid

   #92371

   Purpose

   Inducible expression of M1 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, N184X

   Promoter

   Tet-responsive P tight

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-Tag/SP S245X with 5'UTR

   Plasmid

   #92370

   Purpose

   expression of 245X spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   S245X

   Promoter

   CMV

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-Tag/M87 N184X

   Plasmid

   #92367

   Purpose

   expression of M87 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-460 bp deleted, N184X

   Promoter

   CMV

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Tag/M1 N184X

   Plasmid

   #92365

   Purpose

   expression of M1 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, N184X

   Promoter

   CMV

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-Tag 3B/myc-M87L195V

    Plasmid

    #87721

    Purpose

    Expresses myc-M87L195V spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M87 isoform (deletion of nt 1-490 - 5'UTR an…

    Promoter

    CMV

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-Tag/S46X without 5'UTR

    Plasmid

    #87730

    Purpose

    Expresses S46X spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Expression

    Mammalian

    Mutation

    S46X; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Tag/S44L without 5'UTR

    Plasmid

    #87728

    Purpose

    Expresses S44L spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Expression

    Mammalian

    Mutation

    S44L; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-Tag/L195V without 5'UTR

    Plasmid

    #87726

    Purpose

    Expresses L195V spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Expression

    Mammalian

    Mutation

    L195V; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Tag/E112K without 5'UTR

    Plasmid

    #87724

    Purpose

    Expresses E112K spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Expression

    Mammalian

    Mutation

    E112K; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-Tag 3B/myc-M87E112K

    Plasmid

    #87720

    Purpose

    Expresses myc-M87E112K spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M87 isoform (deletion of nt 1-490 - 5'UTR an…

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hAAVS1-gs4_EF1a-Cas9-2A-GFP

    Plasmid

    #118414

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA AAVS1 (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mApple-M1 Spastin

    Plasmid

    #134461

    Purpose

    M1 Spastin expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Expression

    Mammalian

    Mutation

    Met to Ala change at amino acid 87

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDG458

    Plasmid

    #100900

    Purpose

    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX458-Ef1a-Cas9-H2B-mCherry

    Plasmid

    #159655

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMGS7 (AAVS1sgRNA)

    Plasmid

    #126582

    Purpose

    A CRISPR/Cas9 construct to target the AAVS1 locus in humans

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    AAVS1 sgRNA
    

    Expression

    Mammalian

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge