Addgene

We narrowed to 9,727 results for: ada

Showing: 881 - 900 of 9727 results

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pBbS5c-mCherry-AsLOV2*(543)

Plasmid

#202712

Purpose

Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

Depositor

Mary Dunlop

Article

Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

Insert

mCherry-AsLOV2*(543)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40R

Plasmid

#105303

Purpose

To study effects of acetylation-null tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K40R

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-F3

Plasmid

#32634

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

B19N, B19P, B19M and B19L

Use

Tags

Expression

Mammalian

Mutation

In frame insertion of Ser after aa10 in M (matrix…

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pTubulin-Electra2

Plasmid

#184930

Purpose

Tubulin labeling with blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

TUBA1B-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP Tensin3

Plasmid

#105299

Purpose

To study localization and function of Tensin3

Depositor

Kenneth Yamada, David Critchley

Article

Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

Insert

Tensin3 (TNS3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-qmTQ2-ATP

Plasmid

#225248

Purpose

mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTubulin-mTagBFP2

Plasmid

#184931

Purpose

Tubulin labeling with blue fluorescent protein mTagBFP2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

TUBA1B-mTagBFP2

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRFP-NLS

Plasmid

#105296

Purpose

To visualize nuclear dynamics in living cells

Depositor

Kenneth Yamada

Article

Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

Insert

Rac1 (RAC1 Human)

Use

Tags

RFP

Expression

Mammalian

Mutation

aa encoding 172-192

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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AiRTi

Plasmid

#214068

Purpose

Fluorescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Use

Tags

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AiRT

Plasmid

#214067

Purpose

Fluorescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Use

Tags

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y397F

Plasmid

#50503

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Use

Tags

HA

Expression

Mammalian

Mutation

Y397F

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
LRTi

Plasmid

#214072

Purpose

Luminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Use

Tags

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AiRT_RTctrl

Plasmid

#214070

Purpose

Fluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Use

Tags

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y407F

Plasmid

#50504

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Use

Tags

HA

Expression

Mammalian

Mutation

Y407F

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pST44-AP-1 core

Plasmid

#184809

Purpose

Bacterial expression of clathrin adaptor AP-1 core complex

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Hs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1

Use

Tags

GST tag and His6

Expression

Bacterial

Mutation

AP1S3: S148C; AP1B1: K359R, E476K

Promoter

T7

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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LRTX_ctrl

Plasmid

#214073

Purpose

Luminescent reporter system, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Use

Tags

Expression

Mammalian

Mutation

Remnant of linker sequence present at the end of …

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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hfDECOR-F148A

Plasmid

#219546

Purpose

Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

hfDECOR-F148A

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Changed TadA8e Phenylalanine 148 to Alanine

Promoter

CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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hfDECOR-V106G

Plasmid

#219547

Purpose

Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

hfDECOR-V106G

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Changed TadA8e Valine 106 to Glycine

Promoter

CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40A

Plasmid

#105304

Purpose

To study effects of acetylation-null tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K40A

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LRT_RTctrl

Plasmid

#214074

Purpose

Luminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Use

Tags

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge