We narrowed to 9,864 results for: plasmids 101

1. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

   Plasmid

   #169550

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTS2304-Tier1-Tactb-PPGK-NLuc_TB

   Plasmid

   #169549

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MSP1830

   Plasmid

   #70708

   Purpose

   Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Insert

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   CAG

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDSHA-ARL8A-Myc

   Plasmid

   #67361

   Purpose

   Mammalian expression of hArl8 with C-term Myc tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8A (ARL8A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pDONR221-ARL8A

   Plasmid

   #67332

   Purpose

   hArl8a with stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8A (ARL8A Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pDSHA-ARL4C-Myc

   Plasmid

   #67360

   Purpose

   Mammalian expression of hArl4c with C-term Myc tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4C (ARL4C Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pDSHA-ARL4-Myc

   Plasmid

   #67356

   Purpose

   Mammalian expression of hArl4 with C-term Myc tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4 (ARL4A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pDONR221-ARL4

   Plasmid

   #67322

   Purpose

   hArl4 with stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4 (ARL4A Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Bacterial

   Mutation

   bp 105 A to T; silent mutation

   Promoter

   None

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDONR221-ARL4C

   Plasmid

   #67330

   Purpose

   hArl4c with stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL7 (ARL4C Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pDEST14-ARL14

    Plasmid

    #67385

    Purpose

    Expression of native hArl14 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL14 (ARL14 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    bp 172 C to T; aa L58F

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pDEST14-ARFRP1

    Plasmid

    #67387

    Purpose

    Expression of native hArfrp in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARFRP1 (ARFRP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pDEST14-ARL4

    Plasmid

    #67377

    Purpose

    Expression of native hArl4 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4 (ARL4A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    bp 105 A to T; silent mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. TRE-MPRA_minPromega Library

    Pooled Library

    #201013

    Purpose

    Massively parallel reporter assay (MPRA) library with minPromega promoter

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Expression

    Mammalian

    Use

    Luciferase

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

14. TRE-MPRA_minCMV Library

    Pooled Library

    #201012

    Purpose

    Massively parallel reporter assay (MPRA) library with minCMV promoter

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Expression

    Mammalian

    Use

    Luciferase

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

15. TRE-MPRA_minTK Library

    Pooled Library

    #201014

    Purpose

    Massively parallel reporter assay (MPRA) library with minTK promoter

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Expression

    Mammalian

    Use

    Luciferase

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

16. pSN17

    Plasmid

    #100104

    Purpose

    expresses a chimeric OCT4-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to full length HP1 alpha (POU5F1, CBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

    Plasmid

    #160540

    Purpose

    Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

    Inserts

    SARS-CoV-2 NSP7 (ORF1ab Synthetic)
    SARS-CoV-2 NSP8 (ORF1ab Synthetic)
    SARS-CoV-2 NSP12 (ORF1ab Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAAV-Ucp1-Cre-miR122

    Plasmid

    #228408

    Purpose

    Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

    Depositor

    Joerg Heeren

    Article

    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

    Inserts

    cre recombinase with SV40 NLS
    Cre recombinase with SV40 NLS
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Ucp1 enhancer-promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCC336

    Plasmid

    #110167

    Purpose

    miniMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

    Depositor

    Iva Greenwald

    Article

    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

    Insert

    ERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
    

    Use

    Minimos transposon

    Tags

    mCherry and mClover

    Expression

    Worm

    Mutation

    Phospho-acceptor sites mutated: S43E, T55E, T62E.…

    Promoter

    lin-31 promoter/enhancer

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCC335

    Plasmid

    #110166

    Purpose

    miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

    Depositor

    Iva Greenwald

    Article

    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

    Insert

    ERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
    

    Use

    Minimos transposon

    Tags

    mCherry and mClover

    Expression

    Worm

    Mutation

    Phospho-acceptor sites mutated: S43A, T55A, T62A.…

    Promoter

    lin-31 promoter/enhancer

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge