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pDONR221-NPC1

Plasmid

#132295

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

NPC1 (NPC1 Human)

Expression

Mammalian

Available since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-NPC1

Plasmid

#132295

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

NPC1 (NPC1 Human)

Expression

Mammalian

Available since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pET41-MBP-t-A3A-t-His

Plasmid

#109231

Purpose

Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

Depositor

Rahul Kohli

Publication

Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

Insert

human APOBEC3A (APOBEC3A Optimized for E. coli expression, Human)

Tags

His and MBP

Expression

Bacterial

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET41-MBP-t-A3A-t-His

Plasmid

#109231

Purpose

Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

Depositor

Rahul Kohli

Publication

Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

Insert

human APOBEC3A (APOBEC3A Optimized for E. coli expression, Human)

Tags

His and MBP

Expression

Bacterial

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCDNA5-Flag-BRD4-BD

Plasmid

#90005

Purpose

expresses BD mutant BRD4

Depositor

Kornelia Polyak

Publication

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 140N to 140A, 433N to 433A

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCDNA5-Flag-BRD4-BD

Plasmid

#90005

Purpose

expresses BD mutant BRD4

Depositor

Kornelia Polyak

Publication

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 140N to 140A, 433N to 433A

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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IL2Rα-EGFP

Plasmid

#86055

Purpose

A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Interleukin-2 receptor subunit alpha (IL2RA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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IL2Rα-EGFP

Plasmid

#86055

Purpose

A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Interleukin-2 receptor subunit alpha (IL2RA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_PIK3CA_p.E545K

Plasmid

#82881

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_006218 (PIK3CA Human)

Use

Gateway Cloning

Mutation

E545K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDONR223_PIK3CA_p.E545K

Plasmid

#82881

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_006218 (PIK3CA Human)

Use

Gateway Cloning

Mutation

E545K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDONR223_ATG16L1_WT

Plasmid

#82255

Purpose

Gateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATG16L1 (ATG16L1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATG16L1_WT

Plasmid

#82255

Purpose

Gateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATG16L1 (ATG16L1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Cx43-msfGFP

Plasmid

#69007

Purpose

Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

Depositor

David Spray

Publication

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Gja1 (Gja1 Rat)

Tags

monomerized superfolder GFP

Expression

Mammalian

Promoter

cmv

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Cx43-msfGFP

Plasmid

#69007

Purpose

Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

Depositor

David Spray

Publication

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Gja1 (Gja1 Rat)

Tags

monomerized superfolder GFP

Expression

Mammalian

Promoter

cmv

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mRFP-FKBP12-5ptpase domain

Plasmid

#67516

Purpose

Recruitable human type IV 5-phosphatase domain (214-644)

Depositor

Tamas Balla

Publication

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
mRFP-FKBP12-5ptpase domain

Plasmid

#67516

Purpose

Recruitable human type IV 5-phosphatase domain (214-644)

Depositor

Tamas Balla

Publication

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pEF5/FRT/DEST-3xHA/mGli3/Flag

Plasmid

#51246

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C

Depositor

Rajat Rohatgi

Publication

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEF5/FRT/DEST-3xHA/mGli3/Flag

Plasmid

#51246

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C

Depositor

Rajat Rohatgi

Publication

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMXs-IP-EGFP-LC3

Plasmid

#38195

Depositor

Noboru Mizushima

Publication

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMXs-IP-EGFP-LC3

Plasmid

#38195

Depositor

Noboru Mizushima

Publication

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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