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promoter-less mRFP1E

Plasmid

#183144

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1E

Depositor

Anthony Forster

Publication

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2124

Plasmid

#178791

Purpose

Prgef-1 EGL-3 unc-54 3' UTR C.elegans pan-neural expression of EGL-3

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

EGL-3 (egl-3 Nematode)

Expression

Worm

Promoter

Prgef-1

Available since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pJH26_pBad_AraC_Para-sfGFP.1TAG

Plasmid

#163721

Purpose

Arabinose-inducible expression of green fluorescent protein encoding one amber codon

Depositor

Matthew Bennett

Publication

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp.1TAG

Expression

Bacterial

Mutation

gfp.N39TAG

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH773_pTech_PlldR-HHRz-supP(opal)

Plasmid

#163732

Purpose

Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

Depositor

Matthew Bennett

Publication

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

hhrz-supP

Expression

Bacterial

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO CPTP

Plasmid

#170735

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Publication

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMT Draper-mCherry

Plasmid

#126667

Purpose

Inducible expression of Draper-mCherry in S2 cells

Depositor

Ron Vale

Publication

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper mCherry

Expression

Insect

Mutation

C813Y- see depositor comments below

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pucKan_ARS_pgkZeo_ncx1_mCherry_2TA_Puro_ARS

Plasmid

#163755

Purpose

piggybac transposon encoding human Ncx1 gene promoter-driven mCherry and puromycin-acetyltransferase for labelling and selection of pluripotent stem cell-derived cardiomyocytes

Depositor

Kurt Pfannkuche

Publication

Hamad et al Methods Mol Biol. 2021 Oct 19. doi: 10.1

Insert

mCherry

Use

Piccybac transposon

Tags

puromycin-N-acetyltransferase

Expression

Mammalian

Promoter

human NCX1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-ProA18-GCaMP6s

Plasmid

#126004

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GCaMP6s

Use

AAV

Promoter

ProA18

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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S13_EcoRV_UBC_AscI

Plasmid

#128477

Purpose

UBC promoter

Depositor

Lindy Barrett

Publication

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

UBC promoter

Expression

Bacterial

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRS414-ATG41

Plasmid

#113080

Purpose

Contain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.

Depositor

Daniel Klionsky

Publication

Yao et al Autophagy. 2015;11(12):2288-99. doi: 10.

Insert

ATG41

Expression

Bacterial and Yeast

Promoter

ATG41 endogenous promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAP685-2

Plasmid

#99490

Purpose

TEV::eGFP::ER::6xHis::linker::3XFlag

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::6Xhis::linker::3XFlag

Expression

Bacterial

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-LacZ30

Plasmid

#65028

Purpose

GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 3. See Depositor Comments below for information regarding the lacZ sequence.

Depositor

Herbert Schweizer

Publication

Choi et al Nat Protoc. 2006;1(1):153-61.

Type

Empty backbone

Tags

lacZ

Expression

Bacterial

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pICSL80016

Plasmid

#50332

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, beta-glucuronidase (E. coli) with two introns

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICH44157

Plasmid

#50258

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, RbcS2B (AT5g38420, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45125

Plasmid

#50261

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, LHB1B1 (AT2g34430, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 3

Plasmid

#30161

Depositor

Dennis Selkoe, Tracy Young-Pearse

Publication

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 3

Expression

Mammalian

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pENTR APLP2 shRNA 1

Plasmid

#30159

Depositor

Dennis Selkoe, Tracy Young-Pearse

Publication

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 1

Expression

Mammalian

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pENTR APLP1 shRNA 1

Plasmid

#30156

Depositor

Dennis Selkoe, Tracy Young-Pearse

Publication

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP1 shRNA 1

Expression

Mammalian

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
MSCV-PM mir shHoxB9 b

Plasmid

#27020

Depositor

Joan Massague

Publication

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir HoxB9 3b

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pT7RNAP-T15

Plasmid

#199991

Purpose

T7 RNAP circuit variant T15 on miniRK2 plasmid

Depositor

Farren Isaacs

Publication

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Expression

Bacterial

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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