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mCit(S1h-S3h)3-gs

Plasmid

#228076

Purpose

Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, S1h, S3h

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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p54.mAldh1a1.flag.mSrtA-3M.wpre

Plasmid

#226771

Purpose

secretory SortaseA in p52 (mAldh1a backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

sortase A (srtA )

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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mCit(D1h-D3h)3-gs

Plasmid

#228088

Purpose

Mammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, D1h, D3h

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2h)12-gs

Plasmid

#228087

Purpose

Mammalian expression of coiled coil segments (S2h)x12, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2h

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2h)3(S4h)3-gs

Plasmid

#228077

Purpose

Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2h, S4h

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMK459 (dTAG-BromoTag-mAID-EGFP)

Plasmid

#214387

Purpose

dTAG-BromoTag-mAID-EGFP reporter (PiggyBac)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

dTAG-BromoTag-mAID-EGFP reporter

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SOD1

Plasmid

#222568

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOD1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-KDM5B

Plasmid

#222555

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of KDM5B

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

KDM5B (KDM5B Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-3xFLAG-SMAD1-active

Plasmid

#222579

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMAD1 (SMAD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

S487E, S489E

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-TASL

Plasmid

#221265

Purpose

Expression of TASL in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TASL (Tasl Mouse)

Use

Retroviral

Tags

3xFLAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1(W795R)-StayGold

Plasmid

#210822

Purpose

Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (W795R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

StayGold

Expression

Mammalian

Mutation

Changed Trp-795 to Arg

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-TwinStrep-His

Plasmid

#210816

Purpose

Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

TwinStrep, 10xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-StayGold

Plasmid

#210821

Purpose

Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

StayGold

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-ZC3H12B

Plasmid

#221300

Purpose

Expression of ZC3H12B (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ZC3H12B (pLxIS)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TRBP-L326A/V336A/H338A

Plasmid

#215894

Purpose

Expression of TRBP-L326A/V336A/H338A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-L326A/V336A/H338A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TRBP-V336A/H338A/Y358A

Plasmid

#215893

Purpose

Expression of TRBP-V336A/H338A/Y358A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-V336A/H338A/Y358A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TRBP-V336A/H338A

Plasmid

#215891

Purpose

Expression of TRBP-V336A/H338A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-V336A/H338A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH3.1-GS3

Plasmid

#204762

Purpose

Expression of Cas9 and human H3.1

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H3.1 (H3C6 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A-GS3

Plasmid

#204753

Purpose

Expression of Cas9 and human H2A

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2A (H2AC20 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.1-GS3

Plasmid

#204754

Purpose

Expression of Cas9 and human H2A.1

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2A.1 (H2AC17 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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