We narrowed to 23,662 results for: CRISPR

1. pSG1017-2xBsaI-SaKKH-2xUGI_P2A_EGFP

   Plasmid

   #239450

   Purpose

   Codon-optimized S. aureus KKH Cas9 with 2x UGI. 2x BsaI sites for easy cloning of varying deaminases for cytosine base editing. EGFP can serve as a transfection marker.

   Depositor

   Alexis Komor

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSG1040-rA1-SaKKH-2xUGI_P2A_EGFP

   Plasmid

   #239463

   Purpose

   Cytosine base editor with codon-optimized S. aureus KKH Cas9, rAPOBEC1 deaminase, and 2x UGI. EGFP can be used as a transfection marker.

   Depositor

   Alexis Komor

   Insert

   S.a. rA1 CBE 2xUGI
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZNF215-donor

   Plasmid

   #113804

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF215 with GFP

   Depositor

   Kevin White

   Insert

   ZNF215 homology arms flanking EGFP-IRES-Neo cassette (ZNF215 Human)
   

   Use

   CRISPR

   Mutation

   rs2239733, rs2239732, hg38:11:6956348:T>G, rs1…

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZNF41-donor

   Plasmid

   #112375

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF41 with GFP

   Depositor

   Kevin White

   Insert

   ZNF41 homology arms flanking EGFP-IRES-Neo cassette (ZNF41 Human)
   

   Use

   CRISPR

   Mutation

   hg38:X:47447496:G>T, hg38:X:47448269:C>A, h…

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pELF4-donor

   Plasmid

   #112371

   Purpose

   CRISPR donor plasmid to tag human transcription factor ELF4 with GFP

   Depositor

   Kevin White

   Insert

   ELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)
   

   Use

   CRISPR

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZBTB26-donor

   Plasmid

   #112356

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZBTB26 with GFP

   Depositor

   Kevin White

   Insert

   ZBTB26 homology arms flanking EGFP-IRES-Neo cassette (ZBTB26 Human)
   

   Use

   CRISPR

   Mutation

   hg38:9:122919395:A>G, hg38:9:122919458:T>G,…

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZNF84-donor

   Plasmid

   #112330

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF84 with GFP

   Depositor

   Kevin White

   Insert

   ZNF84 homology arms flanking EGFP-IRES-Neo cassette (ZNF84 Human)
   

   Use

   CRISPR

   Mutation

   rs623100, hg38:12:133058872:T>C, hg38:12:13305…

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZNF75A-donor

   Plasmid

   #112307

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF75A with GFP

   Depositor

   Kevin White

   Insert

   ZNF75A homology arms flanking EGFP-IRES-Neo cassette (ZNF75A Human)
   

   Use

   CRISPR

   Mutation

   hg38:16:3317462:A>T, hg38:16:3317715:C>T, h…

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZNF764-donor

   Plasmid

   #112298

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZNF764 with GFP

   Depositor

   Kevin White

   Insert

   ZNF764 homology arms flanking EGFP-IRES-Neo cassette (ZNF764 Human)
   

   Use

   CRISPR

   Mutation

   hg38:16:30555233:G>A, hg38:16:30555234:C>G,…

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72667

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

    Plasmid

    #67991

    Purpose

    CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-PEmax-P2A-EGFP (LM1589)

    Plasmid

    #223136

    Purpose

    pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized PEmax-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP

    Expression

    Mammalian

    Mutation

    M-MLV mutations from PE2 (Anzalone et al. Nature …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196082

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196080

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CROPseq-SFFV-eBFP2

    Plasmid

    #239601

    Purpose

    Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection

    Depositor

    Christian Krebs

    Article

    Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.

    Insert

    spleen focus‑forming virus - promotor
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEasyG1_nat

    Plasmid

    #184911

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-sadCas9-VP64

    Plasmid

    #115790

    Purpose

    An AAV vector expressing S. aureus dCas9 fused to VP64.

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    Sa-dCas9
    

    Use

    AAV

    Tags

    NLS and NLS-VP64

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-U6-sasgRNACMV- mCherry-WPREpA

    Plasmid

    #217015

    Purpose

    S. aureus sgRNAs backbone along with mCherry expression

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TLCV2

    Plasmid

    #87360

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-2A-eGFP
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Cas9-T2A-eGFP

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge