We narrowed to 90,020 results for: Mal

1. MDH1-miR-31-PGK-GFP-Blast

   Plasmid

   #31515

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   miR-31 (Mir31 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

2. DCT.VV.Orf170

   Plasmid

   #12858

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf170
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

   Plasmid

   #174891

   Purpose

   amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

   Plasmid

   #98041

   Purpose

   Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Insert

   Sa-dCas9-NLS-3xFLAG
   

   Use

   CRISPR

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   D10A + H557A

   Promoter

   CMV

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 Ub2-UBB+1

   Plasmid

   #23968

   Depositor

   Nico Dantuma

   Article

   Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

   Insert

   Ubiquitin B +1 transcript (UBB Human)
   

   Expression

   Mammalian

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-hA3F-BE3

   Plasmid

   #113414

   Purpose

   Expresses hA3F-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3F-BE3 (APOBEC3F S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_GWRev_Dest

   Plasmid

   #86954

   Purpose

   Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)

   Depositor

   Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ3 SHP2 CS

   Plasmid

   #8319

   Depositor

   Ben Neel

   Article

   Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

   Insert

   SHP2 (PTPN11 Human)
   

   Expression

   Mammalian

   Mutation

   C459S, mutation of essential catalytic cysteine

   Available Since

   June 17, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG HDAC5 S259A/S498A

   Plasmid

   #32216

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser259Ala, Ser498Ala

   Promoter

   CMV

   Available Since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pCMV6M-Pak1 H83L H86L

    Plasmid

    #12211

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PBD (p21-binding domain) mutant: H83L, H86L (see …

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330.Pten.a

    Plasmid

    #66587

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Expression

    Mammalian

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pRK5 GFP RagB Q99L

    Plasmid

    #112749

    Purpose

    express GFP-RagB (Q99L mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    GFP-RagB (Q99L) (RRAGB Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Q99L

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_GW_Dest

    Plasmid

    #86955

    Purpose

    Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

    Depositor

    Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA GFP10-RHOB

    Plasmid

    #182237

    Purpose

    Expresses RHOB GTPase with tripartite split GFP10 tag

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Insert

    ras homolog family member B (RHOB Human)
    

    Tags

    GFP10 (split-GFP tripartite)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-hSOX2 (Plath)

    Plasmid

    #17965

    Depositor

    Kathrin Plath

    Article

    Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

    Insert

    SRY (sex determining region Y)-box 2 (SOX2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

16. RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

    Plasmid

    #139445

    Purpose

    Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag E2-crimson
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe puro RalA S31N

    Plasmid

    #19718

    Depositor

    Channing Der

    Article

    Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

    Insert

    V-ral simian leukemia viral oncogene homolog A (RALA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    S31N mutant for dominant neg function. NOTE: lo…

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

18. LentiGuide-Puro-GFPg1 (BB09)

    Plasmid

    #139461

    Purpose

    Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    GFP-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTriEx-mCherry-LINXa3

    Plasmid

    #73614

    Purpose

    Light-induced nuclear export switch for microscopy in mammalian cells.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa3
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    Chicken B-Actin

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-LGC-53

    Plasmid

    #86399

    Purpose

    Mammalian expression of codon-optimized dopamine receptor LGC-53

    Depositor

    Steven Mennerick

    Article

    Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

    Insert

    LGC-53 (lgc-53 Synthetic, Nematode)
    

    Expression

    Mammalian

    Mutation

    Codon optimized for mammalian expression; Signal …

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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