169,450 results
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Plasmid#186302PurposeExpresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoterDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only
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VP64-dCas9
Plasmid#177171PurposeVP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertVP64-dCas9(D10A, H840A)
UseCRISPRTagsNLS and NLS-3xFLagExpressionMammalianPromoterCMVAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#60847PurposeExpresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeastDepositorInsertsS. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA
UseCRISPR and Synthetic BiologyTagsNLS/His8 and TTT 3' extension prior to sgRNAExpressionBacterial and YeastMutationL4 is UUCG tetraloop and guide targets LYP1 (CATA…Available SinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB
Plasmid#154473PurposeExpresses dCas9 fused to mCherry and the KRAB domain of ZIM3DepositorInsertZIM3 (ZIM3 Human)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterSFFVAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
35S-sfGFP-nosT
Plasmid#80129PurposeTransient expression vector for sfGFP in plantsDepositorTypeEmpty backboneTagssfGFPExpressionPlantPromoterCaMV35SAvailable SinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pb-TRE3G-SpCas9-IRES-blast
Plasmid#195506PurposeInducible Piggybac vector expressing SpCas9 with Blasticidin selectionDepositorInsertspCas9
UseCRISPRExpressionMammalianPromoterTREAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Luciferase-IRES-Blast-WPRE
Plasmid#108542PurposeLentivirus vector expressing Luciferase with blasticidin resistanceDepositorInsertsLuciferase
Neomycin
UseLentiviralPromoterEF1apha and PGKAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAX198
Plasmid#173042PurposeCustom vector for sgRNA library constructionDepositorInsertHBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
UseCRISPR and LentiviralExpressionMammalianPromotermouse U6Available SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL403
Plasmid#119953PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
ExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…Available SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-HPH-C4
Plasmid#160343PurposeHigh-copy expression vector for K. marxianus, hygromycin selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only