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Plasmid#179157PurposepiggyBac plasmid for 5' ENGRAM recorderDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianPromoterminPAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only
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pYPQ134B
Plasmid#179216PurposeGolden Gate entry vector; 4th gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRPromoterAtU3Available SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRE reporter backbone
Plasmid#158678PurposeThis plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberryDepositorTypeEmpty backboneUseLentiviralTagsmStrawberryExpressionMammalianAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 nonmutant
Plasmid#183813PurposeExpress CD74-ROS1 fusion (C6;R34) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianPromoterCMVAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOT_2 - lenti-EFS-tNGFR-2A-puro
Plasmid#181971PurposeExpresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.DepositorAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5155
Plasmid#175391PurposeLow Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMART Tmem192-3X HA (targeting vector for genomic tagging)
Plasmid#175777PurposepSMART Tmem192-3X HA (targeting vector for genomic tagging)DepositorInsertTMEM192 (TMEM192 Human)
UseCRISPRAvailable SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTN4
Plasmid#174212PurposeDonor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locusDepositorAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGFPmAID
Plasmid#174213PurposeGFP::mAID donor backboneDepositorTypeEmpty backboneExpressionWormAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_8xMmaPylT_EF1_FLAG-MmaPylRS
Plasmid#174890PurposeMma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationU25C PylTPromoterEF1Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hAdipoq-GFP
Plasmid#176215PurposeExpresses GFP under the control of distal enhancer and the promoter of human adiponectin geneDepositorInsertGFP
UseAAVExpressionMammalianAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)
Plasmid#174515PurposeRecoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)DepositorInsertsMm PylS
MmtRNA-Pyl-opt(UGA)
ExpressionBacterialMutationASL from the serT gene (UGA anticodon) and recodedPromoterGlnS and lppAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB1678 CMV-HIVNES-GS-Cas13bt1
Plasmid#176316PurposeCMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)DepositorInserthuman codon optimized Cas13bt1
UseCRISPRTagsHIV NESExpressionMammalianPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-Gq-P2A-dTomato (AAV PHP.eB)
Viral Prep#135635-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-S5E2-Gq-P2A-dTomato (#135635). In addition to the viral particles, you will also receive purified pAAV-S5E2-Gq-P2A-dTomato plasmid DNA. Expression of Gq-DREADD and dTomato (physically separate) under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorTagsdTomato (physically separate, not a fusion protein)Available SinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSyn-somBiPOLES-mCerulean
Plasmid#154945PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianPromoterhuman synapsinAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-miniAAVR-FLAG
Plasmid#166717PurposeVector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tagDepositorInsertminiAAVR-FLAG
UseLentiviralTagsFLAGExpressionMammalianMutationOnly expressing PKD1-3 of ectodomainPromoterCMVAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOsU3
Plasmid#170132PurposeFor sgRNA expression in monocotyledons protoplastsDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP (AAV8)
Viral Prep#137153-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP (#137153). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of NpHR3.3-EYFP (inhibited in presence of Flp) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Cre-dependent)Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)
Plasmid#163077PurposeEntry vector for: Peredox NADH/NAD+ sensor (cytosolic)DepositorInsertPeredox
UseGateway CloningTagsmCherryAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-Flex-GFP-Fishell_6 (AAV1)
Viral Prep#83895-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hDlx-Flex-GFP-Fishell_6 (#83895). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-GFP-Fishell_6 plasmid DNA. hDlx-driven, Cre-dependent expression of GFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsGFP (Cre-dependent)Available SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only