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pRSCupSup35GFP

Plasmid

#1087

Depositor

Susan Lindquist

Publication

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Available since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJCSUP35

Plasmid

#1088

Depositor

Susan Lindquist

Publication

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

HIS tag

Expression

Bacterial

Available since

May 26, 2005

Availability

Academic Institutions and Nonprofits only
pRSpuro shID1#6

Plasmid

#19165

Depositor

Joan Massague

Publication

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 1 (ID1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pLenti PGK mKate2-OGT WT (Puro)

Plasmid

#154290

Purpose

Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)

Depositor

Suzanne Walker

Publication

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x FLAG and mKate2

Expression

Mammalian

Mutation

WT

Promoter

PGK

Available since

Dec. 31, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Orai1-myc E106Q MO70

Plasmid

#22754

Depositor

Anjana Rao

Publication

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 E106Q (ORAI1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

E106Q

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-PKM2-H391Y

Plasmid

#122692

Purpose

Express His-tagged PKM2 (H391Y) protein in bacteria.

Depositor

Wen-Ching Wang

Publication

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

H391Y

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET30-2-PHGDH-C234S

Plasmid

#107724

Purpose

Expression of PHGDH C234S in bacteria

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

PHGDH (PHGDH Human)

Expression

Bacterial

Mutation

C234S

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Slp2-a C2B

Plasmid

#40053

Depositor

Fernando Martin-Belmonte

Publication

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a C2B (Sytl2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

C2B domain

Promoter

CMV promoter

Available since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pTH371-SUP45-N58A

Plasmid

#29378

Depositor

Tobias von der Haar

Publication

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

N58A mutant gene including genomic sequence 1432 …

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pTH397-SUP45-I222S

Plasmid

#29388

Depositor

Tobias von der Haar

Publication

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

I222S mutant gene (corresponding to the sup45-2 a…

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBS2E

Plasmid

#55169

Purpose

Empty vector, integration at lacA, ampr, mlsr

Depositor

Thorsten Mascher

Publication

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSET FLIPPi-260n

Plasmid

#13552

Depositor

Wolf Frommer

Publication

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi WT

Tags

ECFP and EYFP

Expression

Bacterial

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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MSCV-N 16E6

Plasmid

#37875

Depositor

Karl Munger

Publication

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 16E6 (E6 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available since

July 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
LCMV:ECFP(loxP)EYFP

Plasmid

#31306

Depositor

Gerhart Ryffel

Publication

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

ECFP(loxP)EYFP

Use

Cre/Lox

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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1037 pGL3 FasL promoter

Plasmid

#9028

Depositor

William Sellers

Publication

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

Fas ligand promoter (FASLG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pX330_human CDK1

Plasmid

#118597

Purpose

One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

Depositor

William Earnshaw

Publication

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

guide RNA against human CDK1

Use

CRISPR

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEX-KG-Itch/2793

Plasmid

#11434

Depositor

Allan Weissman

Publication

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

Itch (Itch Mouse)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-PuroR-T2A-eGFP

Plasmid

#234734

Purpose

Puromycin resistance/eGFP cassette in mammalian cells (transfection marker)

Depositor

David Liu

Publication

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PuroR-T2A-eGFP

Expression

Mammalian

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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wtBxb1

Plasmid

#222337

Purpose

Plasmid for expressing wild type Bxb1 in mammalian cells

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized wild type Bxb1

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_nLightG (AAV9)

Viral prep

#187179-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV_hSyn1_nLightG (#187179). In addition to the viral particles, you will also receive purified pAAV_hSyn1_nLightG plasmid DNA. Synapsin-driven expression of norepinephrine sensor nLightG. These AAV preparations are suitable purity for injection into animals.

Depositor

Tommaso Patriarchi

Publication

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Promoter

human Synapsin-1

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only