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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,271 results for: LEA

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Showing: 9141 - 9160 of 16271 results

  1. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

    Plasmid
    #227086
    Purpose
    Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227086-plasmid-map-sequence-id-451101

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  2. CUP1-∆ssCPY*-mScarletI

    Plasmid
    #221157
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221157-plasmid-map-sequence-id-450959

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  3. CUP1-∆ssCPY*-mNeonGreen

    Plasmid
    #221156
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mNeonGreen
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221156-plasmid-map-sequence-id-450957

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  4. pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG

    Plasmid
    #217324
    Purpose
    Purification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S969A
    Promoter
    polyhedrin
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217324-plasmid-map-sequence-id-438435

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  5. pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

    Plasmid
    #217321
    Purpose
    Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S969A
    Promoter
    polyhedrin
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217321-plasmid-map-sequence-id-433443

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  6. pAc5.1B-EGFP-DmNot3-mut2-dsRNAres_Q

    Plasmid
    #147264
    Purpose
    Insect Expression of DmNot3-mut2-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-mut2-dsRNAres (Not3 Fly)
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147264-plasmid-map-sequence-id-289711

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  11. pAc5.1B-EGFP-DmNot3-F764A-dsRNAres_Q

    Plasmid
    #147261
    Purpose
    Insect Expression of DmNot3-F764A-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-F764A-dsRNAres (Not3 Fly)
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147261-plasmid-map-sequence-id-289708

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  12. pAc5.1B-EGFP-DmNot3-mut1-dsRNAres_Q

    Plasmid
    #147263
    Purpose
    Insect Expression of DmNot3-mut1-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-mut1-dsRNAres (Not3 Fly)
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147263-plasmid-map-sequence-id-289710

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  13. ppie.001.301.W257A

    Plasmid
    #137652
    Purpose
    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.
    Depositor
    Tara Davis, Melissa Jurica
    Insert
    ppie (PPIE Human)
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    001-301-W257A
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137652-plasmid-map-sequence-id-381379

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  14. PRPF4.001.522.K45A.pc4

    Plasmid
    #137648
    Purpose
    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    NLS and myc-(His)6
    Expression
    Mammalian
    Mutation
    001-522-K45A-pc4
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137648-plasmid-map-sequence-id-381373

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  15. PRPF4.001.522.Y32A.pc4

    Plasmid
    #137646
    Purpose
    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    NLS and myc-(His)6
    Expression
    Mammalian
    Mutation
    001-522-Y32A-pc4
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137646-plasmid-map-sequence-id-381367

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  16. pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q

    Plasmid
    #147262
    Purpose
    Insect Expression of DmNot3-F818A-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-F818A-dsRNAres (Not3 Fly)
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    147262-plasmid-map-sequence-id-355929

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  17. C-BRPF3-8

    Plasmid
    #166516
    Purpose
    scFv of a human scaffold targeting Bromodomain and PHD finger-containing protein 3. Antigen coverage aa 1056-1195 of 1205, PWWP domain.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166516-plasmid-map-sequence-id-328228

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Showing: 9141 - 9160 of 16271 results