We narrowed to 15,196 results for: invs

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HA-MBP-TEVpCS-ADGRV1 CTF-FLAG

Plasmid

#217710

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRV1 (ADGRV1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-5890

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG1 CTF-FLAG

Plasmid

#217699

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG1 (ADGRG1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-382

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG2 CTF-FLAG

Plasmid

#217700

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG2 (ADGRG2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-606

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLINE1ΔORF2-scFvFc

Plasmid

#206021

Purpose

The ORF2 sequence was removed from pLINE1-scFvFc

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLINE1ΔORF1-2-scFvFc

Plasmid

#206022

Purpose

The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f L27A

Plasmid

#204140

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Leucine 27 changed to Alanine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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ALIX(P801G)-mNeonGreen

Plasmid

#191192

Purpose

Full-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (P801G) (PDCD6IP Human, Synthetic)

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Pro 801 mutated to Gly

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-BT-FLAG

Plasmid

#205621

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted BT domain

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

Plasmid

#205603

Purpose

Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

γ-tubulinWT-RNAi-resistant-GFP

Tags

GFP

Expression

Mammalian

Mutation

G2228A, C2231T, C2237T, G2240A

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

Plasmid

#205604

Purpose

Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

γ-tubulinΔGTP-RNAi-resistant-GFP

Tags

GFP

Expression

Mammalian

Mutation

G2228A, C2231T, C2237T, G2240A, N229A

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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TEG +Q111T

Plasmid

#187435

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed glutamine at 117 to threonine

Promoter

PH and p10

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-basic

Plasmid

#196882

Purpose

Used for preparation of double-floxed inverse ORF vectors

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Type

Empty backbone

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BBLF2/3

Plasmid

#195831

Purpose

Gateway Entry Clone of Epstein-Barr virus BBLF2/3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BBLF2/3 (BBLF2/BBLF3 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BcRF1(vTBP)

Plasmid

#195832

Purpose

Gateway Entry Clone of Epstein-Barr virus BcRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BcRF1 (BcRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BCRF1(vIL10)

Plasmid

#195777

Purpose

Gateway Entry Clone of Epstein-Barr virus BCRF1, vIL-10

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BCRF1, vIL-10 (BCRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BaRF1(RNR-s)

Plasmid

#195795

Purpose

Gateway Entry Clone of Epstein-Barr virus BaRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BaRF1 (BaRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-DrCavin1b

Plasmid

#194292

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJ

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

Multisite gateway entry vector

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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MON +T111H

Plasmid

#187432

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed threonine at 117 to histidine

Promoter

PH and p10

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-K2P4.1-StrepTagII

Plasmid

#191474

Purpose

Expresses K2P4.1 with C-terminal StrepTagII

Depositor

Arthur Laganowsky

Article

Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

Insert

K2P4.1 (KCNK4 Human)

Expression

Insect

Mutation

N104Q N108Q

Promoter

polH

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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TEG +H122D

Plasmid

#187434

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed histidine at 128 to aspartic acid

Promoter

PH and p10

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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