Addgene

We narrowed to 4,720 results for: PGK

Showing: 901 - 920 of 4720 results

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MXW-PGK-IRES-EGFP

Plasmid

#37648

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

mPGK

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

Plasmid

#200475

Purpose

Lentiviral vector expressing gRNA targeting GFP

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W

Plasmid

#208548

Purpose

Lentiviral vector expressing gRNA targeting human GATA3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GATA3(13) (GATA3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-W

Plasmid

#208547

Purpose

Lentiviral vector expressing gRNA targeting human GATA3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GATA3(12) (GATA3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pF hPGK mc2 IRES hygro

Plasmid

#206238

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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LLP249 pGK-dCas9-DNMT3a V3

Plasmid

#100938

Purpose

dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)

Use

CRISPR and Lentiviral

Tags

3xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-blast

Plasmid

#167818

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCCLc-U6-shHMGA2.3-PGK-dTomato

Plasmid

#89606

Purpose

Silencing Hmga2 in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

shRNA targeting HMGA2 (HMGA2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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PLEX-PGK-ANXA11-mCerulean

Plasmid

#164214

Purpose

pLEX lentivirus backbone expresses mCerulean tagged ANXA11 under PGK promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mutation

Promoter

PGK

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP1A-WT-neo

Plasmid

#156173

Purpose

Lentiviral vector for expression of RAP1A-WT, with neomycin section

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-GFPGO-PGK-Neo

Plasmid

#136890

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO

Use

Lentiviral

Tags

Expression

Mutation

ATG>ACG

Promoter

SFFV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti PGK OGT (Neo)

Plasmid

#154289

Purpose

Lentiviral vector encoding full length wildtype human OGT

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

Plasmid

#67983

Purpose

Cas9 activity reporter (control) with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE, modified BFP

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SOX2_v32_7-4)-PGKpuroBFP-W

Plasmid

#211986

Purpose

Express gRNA against SOX2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting SOX2 (SOX2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SOX2_v32_7-5)-PGKpuroBFP-W

Plasmid

#211987

Purpose

Express gRNA against SOX2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting SOX2 (SOX2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

Plasmid

#105039

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.blast-Flag-MFF-S155,172D

Plasmid

#74442

Purpose

Lentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Asp, Ser 172 Asp (Numbering based on MFF …

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only
pCCLc-U6-shHMGA2.2-PGK-dTomato

Plasmid

#89605

Purpose

Silencing Hmga2 in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

shRNA targeting HMGA2 (HMGA2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pF hPGK mc2 IRES neo

Plasmid

#206236

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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PITX3-2A-eGFP-PGK-Puro

Plasmid

#31943

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

PITX3 (PITX3 Human)

Use

Targeting donor

Tags

eGFP

Expression

Mutation

Promoter

-

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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