We narrowed to 2,141 results for: Pam

1. AA301

   Plasmid

   #216030

   Purpose

   Fragmid fragment: (Cas protein) NNGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Sauri]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTBL437 AAVS1 EQR sgRNA

   Plasmid

   #126447

   Purpose

   To cut the human AAVS1 locus for integration.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human AAVS1 safe-harbor locus with NGAG PAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. F11-R pEBio

   Plasmid

   #61486

   Purpose

   Expresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequence

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   F11R (F11R Human)
   

   Use

   Tags

   enzymatic biotinylation sequence and rat CD4 d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. XLone-Fully-spliced CRNDE

   Plasmid

   #242178

   Purpose

   Express doxycycline inducible Fully-spliced CRNDE in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

   Insert

   Fully-spliced CRNDE (CRNDE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PAM mutation (CCT to GTT)

   Promoter

   TRE3GS

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. XLone-Unspliced CRNDE

   Plasmid

   #242177

   Purpose

   Express doxycycline inducible Unspliced CRNDE in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

   Insert

   Unspliced CRNDE (CRNDE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PAM mutation (CCT to GTT)

   Promoter

   TRE3GS

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSIP103: pTarget(CyCAST)

   Plasmid

   #200849

   Purpose

   Target plasmid containing CyPSP1 and attachement site for CyCAST.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Inserts

   PAM for CyCAST + CyPSP1
   tRNA-Leu
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTBL436 HEK293site3EQR sgRNA

   Plasmid

   #126445

   Purpose

   To cut the human HEK293 site3 locus for integration.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human HEK293 site3 with NGAG PAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p35S::SISOBIR1-YFPc

   Plasmid

   #86164

   Purpose

   SOBIR1 split YFP

   Depositor

   Silke Robatzek

   Article

   Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

   Insert

   SOBIR1 (SOBIR1 Mustard Weed)
   

   Use

   Tags

   YFPc

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. p35S::AtSOBIR1-YFPn

   Plasmid

   #86165

   Purpose

   SOBIR1 split YFP

   Depositor

   Silke Robatzek

   Article

   Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

   Insert

   SOBIR1 (SOBIR1 Mustard Weed)
   

   Use

   Tags

   YFPn

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCBE3-NG

    Plasmid

    #218159

    Purpose

    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Tags

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / L1111…

    Promoter

    rpsL promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PBE-NG

    Plasmid

    #229045

    Purpose

    Modified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.

    Depositor

    Huawei Zhang

    Article

    Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. VE-cad KI gRNA1

    Plasmid

    #92310

    Purpose

    CRISPR-GFP-gRNA for cutting VEcad

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad KI gRNA1 (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. VE-cad KI gRNA2

    Plasmid

    #92311

    Purpose

    CRISPR-GFP-gRNA for cutting VEcad

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad KI gRNA2 (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXs_FLAG-MCART1

    Plasmid

    #133247

    Purpose

    Express FLAG-tagged MCART1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART1 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells and…

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AA025

    Plasmid

    #216005

    Purpose

    Fragmid fragment: (Cas protein) NG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-NG_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AA180

    Plasmid

    #216023

    Purpose

    Fragmid fragment: (Cas protein) NGRR(T) PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Sa]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AA027

    Plasmid

    #216007

    Purpose

    Fragmid fragment: (Cas protein) nearly PAM-less

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-SpRY_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AA026

    Plasmid

    #216006

    Purpose

    Fragmid fragment: (Cas protein) NG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-SpG_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXs_MCART1

    Plasmid

    #133253

    Purpose

    Express untagged MCART1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART1 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells and…

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pegRNA-HEK3_del1-5-NGG

    Plasmid

    #185478

    Purpose

    pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    HEK3_del1-5-NGG pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge