We narrowed to 2,171 results for: Pam

1. xCas9

   Plasmid

   #138565

   Purpose

   Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   xCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHEE901-A3A

   Plasmid

   #229044

   Purpose

   nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

   Depositor

   Huawei Zhang

   Article

   Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. p35S::AtSOBIR1-mCherry

   Plasmid

   #86166

   Purpose

   SOBIR1-mCherry expression

   Depositor

   Silke Robatzek

   Article

   Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

   Insert

   SOBIR1 (SOBIR1 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Plant

   Promoter

   35S

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA299

   Plasmid

   #216029

   Purpose

   Fragmid fragment: (Cas protein) NNGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Slug]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA296

   Plasmid

   #216028

   Purpose

   Fragmid fragment: (Cas protein) NNNNCC PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Nme2Cas9_v1.1 [Nme2]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA301

   Plasmid

   #216030

   Purpose

   Fragmid fragment: (Cas protein) NNGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Sauri]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTBL437 AAVS1 EQR sgRNA

   Plasmid

   #126447

   Purpose

   To cut the human AAVS1 locus for integration.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human AAVS1 safe-harbor locus with NGAG PAM
   

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. F11-R pEBio

   Plasmid

   #61486

   Purpose

   Expresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequence

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   F11R (F11R Human)
   

   Tags

   enzymatic biotinylation sequence and rat CD4 d3+4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. XLone-Fully-spliced CRNDE

   Plasmid

   #242178

   Purpose

   Express doxycycline inducible Fully-spliced CRNDE in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

   Insert

   Fully-spliced CRNDE (CRNDE Human)
   

   Expression

   Mammalian

   Mutation

   PAM mutation (CCT to GTT)

   Promoter

   TRE3GS

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. XLone-Unspliced CRNDE

    Plasmid

    #242177

    Purpose

    Express doxycycline inducible Unspliced CRNDE in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

    Insert

    Unspliced CRNDE (CRNDE Human)
    

    Expression

    Mammalian

    Mutation

    PAM mutation (CCT to GTT)

    Promoter

    TRE3GS

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSIP103: pTarget(CyCAST)

    Plasmid

    #200849

    Purpose

    Target plasmid containing CyPSP1 and attachement site for CyCAST.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Inserts

    PAM for CyCAST + CyPSP1
    tRNA-Leu
    

    Expression

    Bacterial

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTBL436 HEK293site3EQR sgRNA

    Plasmid

    #126445

    Purpose

    To cut the human HEK293 site3 locus for integration.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human HEK293 site3 with NGAG PAM
    

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p35S::SISOBIR1-YFPc

    Plasmid

    #86164

    Purpose

    SOBIR1 split YFP

    Depositor

    Silke Robatzek

    Article

    Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

    Insert

    SOBIR1 (SOBIR1 Mustard Weed)
    

    Tags

    YFPc

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p35S::AtSOBIR1-YFPn

    Plasmid

    #86165

    Purpose

    SOBIR1 split YFP

    Depositor

    Silke Robatzek

    Article

    Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

    Insert

    SOBIR1 (SOBIR1 Mustard Weed)
    

    Tags

    YFPn

    Expression

    Plant

    Promoter

    35S

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. NG-ABE-NW

    Plasmid

    #240622

    Purpose

    Adenine base editor with narrowed editing window, recognizing NG PAM

    Depositor

    Tingting Jiang

    Article

    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

    Insert

    TadA8e(N108Q, M151C, Q154Y)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    TadA8e(N108Q, M151C, Q154Y), Cas9 (D10A, L1111R, …

    Available Since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. NG-ABE9

    Plasmid

    #240625

    Purpose

    Adenine base editor with narrowed editing window, recognizing NG PAM

    Depositor

    Tingting Jiang

    Article

    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

    Insert

    TadA8e (N108Q/L145T)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    TadA8e (N108Q/L145T), Cas9 (D10A, L1111R, D1135V,…

    Available Since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pegRNA-HEK3_del1-5-NGG

    Plasmid

    #185478

    Purpose

    pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    HEK3_del1-5-NGG pegRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. VE-cad KI gRNA1

    Plasmid

    #92310

    Purpose

    CRISPR-GFP-gRNA for cutting VEcad

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad KI gRNA1 (CDH5 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. VE-cad KI gRNA2

    Plasmid

    #92311

    Purpose

    CRISPR-GFP-gRNA for cutting VEcad

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad KI gRNA2 (CDH5 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCBE3-NG

    Plasmid

    #218159

    Purpose

    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / L1111…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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