We narrowed to 1,102 results for: cas12a

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pICSL1P3_crU626

Plasmid

#191778

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Publication

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P4_crU626

Plasmid

#191779

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Publication

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P5_crU626

Plasmid

#191780

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Publication

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P7_crU626

Plasmid

#191782

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Publication

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRC167_Lenti-hU6-AsDR-BsmBI-EF1a-mCherry-2A-Puro

Plasmid

#256633

Purpose

Lentiviral vector for delivery of AsCas12a guides and expressing mCherry using EFS promoter

Depositor

Sidi Chen

Publication

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Type

Empty backbone

Use

Lentiviral

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKT02_MSCV-hU6-BbsI-cPPT-EFS-Cre-mScarlet

Plasmid

#256628

Purpose

Retroviral vector for delivery of AsCas12a guides and expressing Cre and mScarlet using EFS promoter

Depositor

Sidi Chen

Publication

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Type

Empty backbone

Use

Retroviral

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pRK16b_MSCV-hU6P-LbDR-BbsI-cPPT-EFS-mScarlet

Plasmid

#256632

Purpose

Retroviral vector for delivery of LbCas12a guides and expressing mScarlet using EFS promoter

Depositor

Sidi Chen

Publication

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Type

Empty backbone

Use

Retroviral

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pTF-ahpC-rfp

Plasmid

#153035

Purpose

For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

Depositor

Nigel Scrutton

Publication

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

CRISPR array

Expression

Bacterial

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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Humagne Set C

Pooled library

#172650

Purpose

Set C contains best-ranked guides 1 and 4.

Depositor

John Doench, David Root

Publication

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Expression

Mammalian

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only
pKT07_AAV-hU6-AsDR-crTSG1-EFS-Cre-T2A-hNGFR

Plasmid

#256630

Purpose

AAV vector for delivery of AsCas12a crTSG1 guide array and expressing Cre using EFS promoter

Depositor

Sidi Chen

Publication

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Insert

crTrp53, crPten, crApc, crRb1

Use

AAV

Promoter

humanU6, EFS-NS

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKT07_AAV-hU6-AsDR-EFS-Cre-T2A-hNGFR

Plasmid

#256629

Purpose

AAV vector for delivery of AsCas12a guides and expressing Cre and hNGFR using EFS promoter

Depositor

Sidi Chen

Publication

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Type

Empty backbone

Use

AAV

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pC1.530

Plasmid

#205441

Purpose

Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

Depositor

Alistair McCormick

Publication

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

RepB gRNA

Use

Synthetic Biology

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMV_AA089

Plasmid

#216105

Purpose

gRNA expression for Cas12a (guide only)

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

gRNA expression

Use

CRISPR and Lentiviral; Assembled vector

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.391

Plasmid

#203948

Purpose

Level 0 part. Cas12a coding sequence from Francisella novicida in CDS1 position

Depositor

Alistair McCormick

Publication

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

FnCas12a

Use

Synthetic Biology

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_936

Plasmid

#216094

Purpose

Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD46, CD47, CD55, CD81 guides

Use

CRISPR and Lentiviral; Positive controls

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL089

Plasmid

#137897

Purpose

iSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Publication

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL088

Plasmid

#137896

Purpose

iSTU-CRISPR/Cas12a DR-DR structure crRNA insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Publication

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER1-TF1-dsRED

Plasmid

#213044

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Publication

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

cBH

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER2-TF1-dsRED

Plasmid

#213045

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Publication

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

Plasmid

#202760

Purpose

Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

Depositor

Karl Wahlin

Publication

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

huAsCpf1

Expression

Mammalian

Promoter

CMV

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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