We narrowed to 2,317 results for: pET28

1. pET28 sf:em-blue-shift.1

   Plasmid

   #191928

   Purpose

   Encodes a GFP design, sf:em-blue-shift.1

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:em-blue-shift.1
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S Q69P S72A T108V Y145M T167V V224I

   Promoter

   T7

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a LZ LED 4CA-FLAG

   Plasmid

   #199681

   Purpose

   Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper LYCHOS LED 4CA (GPR155 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   C595.604.629.638 of LYCHOS mutated to A

   Promoter

   T7 promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a LZ LED Y551A-FLAG

   Plasmid

   #199680

   Purpose

   Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper LYCHOS LED Y551A (GPR155 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Y551 of LYCHOS mutated to A

   Promoter

   T7 promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28c(plus)-DmAGO1-RC_F

   Plasmid

   #146262

   Purpose

   Bacterial Expression of DmAGO1-RC

   Depositor

   Elisa Izaurralde

   Insert

   DmAGO1-RC (AGO1 Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one non silent mutation R787C compared to sequenc…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28c(plus)-DmGW182_1-539_F

   Plasmid

   #146263

   Purpose

   Bacterial Expression of DmGW182_1-539

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_1-539 (gw Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28 sf:em-blue-shift.2

   Plasmid

   #191919

   Purpose

   Encodes a GFP design, sf:em-blue-shift.2

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:em-blue-shift.2
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S S72A T108I Y145F V150I

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a Cdk2ap1ΔN (MT2B2)-MutTER

   Plasmid

   #178035

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   First N-Terminal 27aa are deleted and Changed T(8…

   Promoter

   T7LacI

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PET28a-cpLumiLuc-amylase sensor

   Plasmid

   #186293

   Purpose

   Expression alpha-amylase sensor in e coli

   Depositor

   Kirill Alexandrov

   Article

   Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

   Insert

   cpLumiLuc-amylase sensor
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PET28a-cpLumiLuc-HSA sensor

   Plasmid

   #186294

   Purpose

   Expression Human serum albumin sensor in e coli

   Depositor

   Kirill Alexandrov

   Article

   Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

   Insert

   cpLumiLuc-HSA sensor
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28_mouse-Caspase-1-H340D_enzymatic-domain

    Plasmid

    #183388

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (AA130-404)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    H340D

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28_canine-Caspase-1/4_enzymatic-domain

    Plasmid

    #183383

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-anticalin (E60amb)-His

    Plasmid

    #168043

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 60 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-sfGFP-DogTag Loop C

    Plasmid

    #171781

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Asp1…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28-sfGFP-SpyTag003 Loop B

    Plasmid

    #171777

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop B
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues A…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-sfGFP-SpyTag003 Loop C

    Plasmid

    #171778

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues A…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-anticalin (E143amb)-His

    Plasmid

    #168046

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 143 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-anticalin (S87amb)-His

    Plasmid

    #168044

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Ser 87 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-anticalin (N116amb)-His

    Plasmid

    #168045

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 116 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-anticalin (N21amb)-His

    Plasmid

    #168041

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 21 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-anticalin (I55amb)-His

    Plasmid

    #168042

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Ile 55 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge