We narrowed to 1,613 results for: sgrna vector

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pMC-Dest-uni-gRNA5-BsaI

Plasmid

#241150

Purpose

Destination vector for mini-Golden subcloning platform; contains universal sgRNA at 5' end

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

Plasmid

#225754

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

Plasmid

#225756

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDD143

Plasmid

#119880

Purpose

Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_dCas9sth1

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

Plasmid

#225755

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMCB306

Plasmid

#89360

Purpose

sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

GFP, puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDCC5

Plasmid

#59984

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Insert

Cas9-D10A

Use

CRISPR

Tags

HA

Expression

Insect

Mutation

D10A

Promoter

hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pSpyB

Plasmid

#220186

Purpose

sgRNA expression vector - pBG35 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG35

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only
pJF131B

Plasmid

#113326

Purpose

pTet-W108 scRNA.b2, pTet-RR2-sgRNA

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

W108 scRNA_MS2 and sgRNA_RR2

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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ptetgRNA-FRT

Plasmid

#243872

Purpose

sgRNA expression vector under tetR transcriptional control.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

sgRNA_FRT

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pSpy

Plasmid

#220184

Purpose

sgRNA expression vector - promoterless backbone

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

promoterless backbone

Use

CRISPR

Expression

Bacterial

Promoter

promoterless

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyA

Plasmid

#220185

Purpose

sgRNA expression vector - pEM7 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pEM7 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pEM7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyC

Plasmid

#220187

Purpose

sgRNA expression vector - pBG42 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG42

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyD

Plasmid

#220188

Purpose

sgRNA expression vector - pBG28 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG28 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG28

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-Z-SpCas9-gRNA-repeat-polyT-11

Plasmid

#197781

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

gRNA repeat for U6 sgRNA assembly

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA_CV2

Plasmid

#167165

Purpose

pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.

Depositor

Alexander Loewer

Article

Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast ACSL4

Plasmid

#173164

Purpose

Retroviral vector to express sgRNA resistant human ACSL4

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)

Use

Retroviral

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available Since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FKBP12-Gag(HIV)

Plasmid

#138476

Purpose

Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

HIV Gag

Tags

FRBP12 and Lynn Myristolation site

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast mAtg7

Plasmid

#173170

Purpose

Retroviral vector to express sgRNA resistant mouse Atg7

Depositor

Kivanc Birsoy

Article

Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

Insert

Autophagy Related 7 (Atg7 Mouse)

Use

Retroviral

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available Since

Feb. 2, 2022

Availability

Academic Institutions and Nonprofits only

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