We narrowed to 64,738 results for: cell;

1. pScalps_Puro_mTet2 catalytic domain

   Plasmid

   #79554

   Purpose

   Expression of catalytic domain of mouse Tet2

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Tet2 (Tet2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Mutation

   aa990-1912

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-MMLV-gag-hMLH1dn

   Plasmid

   #195513

   Purpose

   MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn (MLH1 Human)
   

   Use

   Virus like particle

   Tags

   FLAG, NES, and Protease cleavate site

   Expression

   Mammalian

   Mutation

   Human MLH1 del754-756

   Promoter

   CMV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScalps_Puro_mTet2 catalytic domain HxD

   Plasmid

   #79611

   Purpose

   Expression of catalytically inactive mouse Tet2

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Tet2 (Tet2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Mutation

   Mutant Tet2 with H1302Y, D1304A substitutions in …

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. EGFP-COV M

   Plasmid

   #165124

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   COV M (M codon optimized SARS-COV-2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCrisprV2 sgRNA hCRY1 c-term

   Plasmid

   #179453

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human CRY1
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX307 P2A GFP YAP1

   Plasmid

   #193675

   Purpose

   Constitutive lentiviral expression of YAP1

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDB-hCRY1-mScarlet-I-CD4-bla

   Plasmid

   #179442

   Purpose

   Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mScarletI
   

   Use

   CRISPR

   Tags

   His/Flag

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-Tet-On shYAP1-9

   Plasmid

   #193668

   Purpose

   Tet inducible knockdown of YAP1

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUW-tetO-loxP-hNANOG

   Plasmid

   #60849

   Purpose

   Lentiviral DOX-inducible expression vector for human NANOG

   Depositor

   Rudolf Jaenisch

   Article

   Theunissen et al Cell Stem Cell. 2014 Oct 2;15(4):471-87.

   Insert

   hNANOG (NANOG Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

    Plasmid

    #164131

    Purpose

    Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-PGK-scFv GCN4-sfGFP-VP64

    Plasmid

    #122133

    Purpose

    Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    scFv GCN4-sfGFP-VP64
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIBN-EYFP-hLaminB1

    Plasmid

    #103800

    Purpose

    BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hLaminB1 (CIB1, LMNB1 Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12

    Plasmid

    #101285

    Purpose

    Lentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFP

    Depositor

    Alpha Yap

    Article

    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

    Insert

    E-Cadherin-GFP-H-Ras
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H-Ras V12

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD649-HAsp-SEMA3F-COMP5AP-AviTag-9xHis

    Plasmid

    #157541

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3F (SEMA3F Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIP TRE-BI GFP10-RHOA/RBD-GFP11

    Plasmid

    #182232

    Purpose

    Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Inserts

    ras homolog family member A (RHOA Human)
    rhotekin RHO binding domain (RBD)
    

    Use

    Lentiviral

    Tags

    GFP10 (split-GFP tripartite) and GFP11 (M4)

    Promoter

    TRE bidirectional

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis

    Plasmid

    #157534

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3E (SEMA3E Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSMPUW-miR-124-GFP-Puro

    Plasmid

    #117321

    Purpose

    Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    hsa-miR-124-1 (MIR124-1 Human)
    

    Use

    Lentiviral

    Tags

    GFP-Puro

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-EGFP MASTL WT (siRNA resistant)

    Plasmid

    #191011

    Purpose

    Expresses EGFP-tagged MASTL WT with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

    Depositor

    Johanna Ivaska

    Article

    Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Microtubule-associated serine/threonine kinase like (MASTL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiV_Neo_POU2F3_CR

    Plasmid

    #122175

    Purpose

    Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

    Insert

    POU2F3 (POU2F3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    3 silent mutations for gRNA resistance (882g-c, 8…

    Promoter

    EFS

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO-Tet-On shYAP1-3

    Plasmid

    #193666

    Purpose

    Tet inducible knockdown of YAP1

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge