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SGK1 gRNA (BRDN0001145161)

Plasmid

#77460

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SGK1 (SGK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ICK gRNA (BRDN0001146785)

Plasmid

#77412

Purpose

3rd generation lentiviral gRNA plasmid targeting human ICK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ICK

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ICK gRNA (BRDN0001148893)

Plasmid

#77413

Purpose

3rd generation lentiviral gRNA plasmid targeting human ICK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ICK

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ICK gRNA (BRDN0001146903)

Plasmid

#77414

Purpose

3rd generation lentiviral gRNA plasmid targeting human ICK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ICK

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SH3BP4 gRNA (BRDN0001149529)

Plasmid

#78087

Purpose

3rd generation lentiviral gRNA plasmid targeting human SH3BP4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SH3BP4 (SH3BP4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SH3BP4 gRNA (BRDN0001487091)

Plasmid

#78089

Purpose

3rd generation lentiviral gRNA plasmid targeting human SH3BP4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SH3BP4 (SH3BP4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-382-Decoy.hPGK.GFP.WPRE

Plasmid

#46618

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-382 Decoy (MIR382, Mir382 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-496-Decoy.hPGK.GFP.WPRE

Plasmid

#46622

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-496 Decoy (MIR496, Mir496a Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
3625 pSP72 Bim (genomic)

Plasmid

#8805

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2004 Oct 26;101

Insert

Bim genomic (Bcl2l11 Mouse)

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pSTTa-GPA1

Plasmid

#248648

Purpose

Vector for protein expression of 2x StrepII-tagged Arabidopsis heterotrimeric G protein GPA1 in E. coli.

Depositor

Sarah Assmann

Article

Gookin et al Front Mol Biosci. 2025 Apr 7;12:1513660.

Insert

GPA1 (GP ALPHA 1 Mustard Weed)

Tags

2x StrepII, Thrombin site, TEV site

Expression

Bacterial

Promoter

tac

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM-7zf(+)_mmSRA1

Plasmid

#232032

Purpose

in vitro transcription of murine Sra1

Depositor

Primo Schaer

Article

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Insert

Sra1 (Sra1 Mouse)

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM-3zf(+)_hsSRA1

Plasmid

#232033

Purpose

in vitro transcription of human Sra1

Depositor

Primo Schaer

Article

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Insert

Sra1 (SRA1 Human)

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_WT-LGP2 (codon optimized)

Plasmid

#248163

Purpose

Expresses full-length human LGP2 in E. coli

Depositor

Smita Patel

Article

Lee et al Nucleic Acids Res. 2024 Jan 11;52(1):355

Insert

DExH-box helicase 58 (DHX58) (DHX58 Human)

Tags

6xHis, SUMO

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pBacPAK9_WT-LGP2

Plasmid

#248165

Purpose

Expresses full-length human LGP2 in sf9 insect cells

Depositor

Smita Patel

Article

Lee et al Nucleic Acids Res. 2024 Jan 11;52(1):355

Insert

DExH-box helicase 58 (DHX58) (DHX58 Human)

Tags

6xHis

Expression

Insect

Promoter

Polyhedrin

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT_superDn29-P2A-GFP

Plasmid

#247162

Purpose

In vitro transcription of human codon optimized engineered superDn29-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

superDn29-P2A-GFP

Use

In vitro transcription

Tags

6xHis

Mutation

M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT_hifiDn29-P2A-GFP

Plasmid

#247164

Purpose

In vitro transcription of human codon optimized engineered hifiDn29-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-P2A-GFP

Use

In vitro transcription

Tags

6xHis

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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SSH-3 WT

Plasmid

#245957

Purpose

Expression of wild-type slingshot 3, a cofilin pSer3 phosphatase

Depositor

James Bamburg, Kensaku Mizuno, Kazumasa Ohashi

Article

Ohta et al Genes Cells. 2003 Oct;8(10):811-24. doi:

Insert

Slingshot protein phosphatase 3 (Ssh3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2CR-QUAS:mNeonGreen-NES-P2A-mKate2-NLS

Plasmid

#241912

Purpose

Zebrafish transgenesis plasmid encoding cytoplasmic mNeonGreen-NES and mKate2-NLS separated by the P2A peptide, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker

Depositor

Balázs Enyedi

Article

Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

Insert

QUAS enhancer driving mNeonGreen-NES and mKate2-NLS

Use

Zebrafish tol2 transgenesis

Promoter

QUAS

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-QUAS:cPla2-mKate2

Plasmid

#241913

Purpose

Zebrafish transgenesis plasmid encoding D. rerio cPla2-mKate2, driven by the QUAS enhancer, and including a cmlc2:EGFP cardiac transgenesis marker

Depositor

Balázs Enyedi

Article

Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

Insert

QUAS enhancer driving cPla2-mKate2

Use

Zebrafish tol2 transgenesis

Tags

cPla2 fused to mKate2

Promoter

QUAS

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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SpG_ATH51-IP

Plasmid

#242074

Purpose

Expresses Cas9-SpG_ATH51 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpG_ATH51

Use

CRISPR

Expression

Mammalian

Mutation

ectopic insertion of 7 amino acids of PID turn-he…

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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