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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,025 results for: 158

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Showing: 9261 - 9280 of 11025 results

  2. pCBS-4559

    Plasmid
    #249331
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) in mammalian cells and gfp-dropout cassette for cloning sgRNAs
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249331-plasmid-map-sequence-id-497004

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  3. pCBS-5391

    Plasmid
    #249330
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and Non-targeting guide for mammalian cells
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249330-plasmid-map-sequence-id-497003

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  4. pCBS-5846

    Plasmid
    #249329
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A)and sg#3 targeting VEGFA gene in mammalian cells
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249329-plasmid-map-sequence-id-497002

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  5. pCBS-6756

    Plasmid
    #249328
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and sg#2 targeting PTEN gene in mammalian cells
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249328-plasmid-map-sequence-id-497001

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  7. pCBS-4803

    Plasmid
    #249321
    Purpose
    Expresses an sgRNA to target the KanR* site on the engineered E. coli MG1655 strain and contains the Repair Template to replace the KanR* on the genome of E. coli MG1655
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    sgRNA targeting the KanR* site
    Expression
    Bacterial
    Mutation
    N/A
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249321-plasmid-map-sequence-id-496999

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  8. pCBS-5738

    Plasmid
    #249324
    Purpose
    To express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(DLAA)-nScCas9(D10A)
    Expression
    Yeast
    Mutation
    DarT2(G49D, M86L, R92A, R193A) and nScCas9(D10A)
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249324-plasmid-map-sequence-id-496429

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  18. pBI121::smRatioGFP:AQ

    Plasmid
    #240453
    Purpose
    Expression of indicator protein fusion (soluble modified ratiometric pHluorin & Aequorin) for monitoring calcium concentrations and pH in the cytoplasm of higher plants. See Resource Information.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of soluble modified ratiometric pHluorin and Aequorin
    Expression
    Bacterial and Plant
    Mutation
    Ratiometric GFP (ratiometric pHluorin) (PMID 9671…
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available Since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240453-plasmid-map-sequence-id-485325

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Showing: 9261 - 9280 of 11025 results