We narrowed to 10,272 results for: virus

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pLV hUbC-Cas9-P2A-Puro_ST-sgRNA

Plasmid

#188705

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting loci

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

ST sgRNAs

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV51

Plasmid

#188484

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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TDRD3:ABC006-C02:C220502

Plasmid

#183982

Purpose

Baculovirus expression for structure determination. May not contain entire coding region of gene.

Depositor

Cheryl Arrowsmith

Insert

TDRD3-anti-TDRD3 FAB (TDRD3 Human)

Tags

anti-TDRD3 FAB HC: N-terminal STII leader peptid…

Expression

Bacterial

Available Since

June 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r

Plasmid

#162936

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Triple FLAG epitope tag fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

Plasmid

#162937

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r

Plasmid

#162935

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Maltose-binding protein fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

Plasmid

#162932

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 fusion with AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GP826

Plasmid

#164551

Purpose

Expresses Smo-FLAG K444, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GP825

Plasmid

#164550

Purpose

Expresses Smo-FLAG K434, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GP824

Plasmid

#164549

Purpose

Expresses Smo-FLAG K434, 444R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 444R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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PD30

Plasmid

#164543

Purpose

Expresses Drd1-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Dopamine receptor D1 (Drd1 Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP807

Plasmid

#164535

Purpose

Expresses Smo noK-FLAG-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG_Ub noK

Mutation

All 21 cytoplasmic Lysines of Smoothened and all…

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-GFPGO2(NGC)-PGK-Neo

Plasmid

#136893

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO2(NGC)

Use

Lentiviral

Tags

2xNLS

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL-GFPGO2(NGT)-PGK-Neo

Plasmid

#136892

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO2(NGT)

Use

Lentiviral

Tags

2xNLS

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Myo 9 Avi+Flag

Plasmid

#134969

Purpose

Expression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1

Depositor

Martin Bähler

Article

Liao et al J Biol Chem. 2010 Aug 6;285(32):24933-42

Insert

Myo 9 (hum-7 Nematode)

Tags

Avi and Flag

Expression

Insect

Promoter

Polyhedrin

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFB HTB_hMSH6_T1219D

Plasmid

#135466

Purpose

Expression human MSH6_T1219D mutant protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6_T1219D (MSH6 Human)

Tags

His-tag

Expression

Insect

Mutation

changed threonine to aspartic acid

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Laforin Myc 3CS (C250S,C266S,C278S)

Plasmid

#84661

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc 3CS_new (C266S, C278S, C329S)

Plasmid

#84662

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pAcUW21-hovimeh1

Plasmid

#84211

Purpose

baculovirus transfer vector containing hovimeh1 coding sequence from Homalodisca vitripennis

Depositor

Bruce Hammock

Article

Kamita et al Arch Insect Biochem Physiol. 2013 Aug;83

Insert

meh1

Expression

Insect

Promoter

p10

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
FLAG-SpEAF pBacPAK8

Plasmid

#15319

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

ELL associated factor (eaf1 Fission Yeast)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only