We narrowed to 14,052 results for: crispr grnas

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pYPQ148

Plasmid

#69300

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 8 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ147

Plasmid

#69299

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL1P2_crU626

Plasmid

#191777

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P7_crU626

Plasmid

#191782

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAC1819_pCR8-DR-SMN2-2-DR

Plasmid

#118653

Purpose

Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-SMN2-2-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL1P6_crU626

Plasmid

#191781

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P3_crU626

Plasmid

#191778

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P4_crU626

Plasmid

#191779

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P5_crU626

Plasmid

#191780

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pUCSh3-mRFP

Plasmid

#170983

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAC1820_pCR8-DR-RG6-SA-DR

Plasmid

#118654

Purpose

Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-RG6-SA-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA1

Plasmid

#170088

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRC065d

Plasmid

#225979

Purpose

dRxCas13d expressed from J23107 promoter with J23119-driven crRNA (non-cognate DR)

Depositor

Jesse Zalatan

Article

Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

Inserts

dRxCas13d
buj1 crRNA (non-cognate DR) targets the unstructured RBS

Use

CRISPR

Promoter

J23107 and J23119

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pUCSh2-mRFP

Plasmid

#170982

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA2

Plasmid

#170089

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_ect2_cmlc2-nmKate

Plasmid

#238410

Purpose

Drives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting ect2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_tnnt2a_cmlc2-nmKate

Plasmid

#238309

Purpose

Drives expression of 3 different gRNAs targeting tnnt2a, and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-ABE C-terminal

Plasmid

#137178

Purpose

AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

CMV

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-CBE C-terminal

Plasmid

#137176

Purpose

AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

Cbh

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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