Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,731 results for: plasmids pcas

Clear keywords
Showing: 921 - 940 of 1731 results

  3. pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

    Plasmid
    #175259
    Purpose
    Dual plasmid labeling membrane target protein of different neuronal population with different fluorophore
    Depositor
    Frank Bradke, Sebastián Dupraz
    Article
    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37
    Insert
    Lox-rox-STOP-rox-lyn-mScarlet-pA-lox
    Use
    Cre/Lox; Dre/rox
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    pCAG
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    175259-plasmid-map-sequence-id-375583

    Enlarge

  5. pCAG-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1060)

    Plasmid
    #114093
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114093-plasmid-map-sequence-id-230492

    Enlarge

  6. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

    Enlarge

  7. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107942-plasmid-map-sequence-id-259760

    Enlarge

  9. pCAGEN-ActinVhh-sfGFP-P2A-HA-tdiRFP-caax (JDW 1532)

    Plasmid
    #242606
    Purpose
    A CAGGS driven expression vector containing an actin nanobody fused to sfGFP to label actin followed by a HA tagged tdiRFP fused to a caax tag to label the cell membrane.
    Depositor
    Joshua Wythe
    Article
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    Actin Vhh-sfGFP-P2A-HA-tdiRFP-caax
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242606-plasmid-map-sequence-id-495400

    Enlarge

  12. pCAG-lox-inv[Talpha1-Dre-PA]-lox-2xHA-CAMSAP3

    Plasmid
    #196895
    Purpose
    Neuron-specific expression of Calmodulin Regulated Spectrin Associated Protein Family Member 3 (CAMSAP3) fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    2xHA-CAMSAP3 (Camsap3 Mouse)
    Use
    Cre/Lox; Dre/rox
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196895-plasmid-map-sequence-id-392230

    Enlarge

  15. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

    Plasmid
    #107300
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP
    Use
    CRISPR
    Tags
    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107300-plasmid-map-sequence-id-204631

    Enlarge

  16. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

    Plasmid
    #107299
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP
    Use
    CRISPR
    Tags
    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107299-plasmid-map-sequence-id-204615

    Enlarge

  17. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid
    #114085
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114085-plasmid-map-sequence-id-230653

    Enlarge

  18. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

    Plasmid
    #114086
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    114086-plasmid-map-sequence-id-231034

    Enlarge

  19. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

    Plasmid
    #114080
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114080-plasmid-map-sequence-id-230482

    Enlarge

  20. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

    Plasmid
    #114079
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114079-plasmid-map-sequence-id-231032

    Enlarge

Showing: 921 - 940 of 1731 results