We narrowed to 1,480 results for: FLP

1. pcDNA5_NLS-Halo-eGFP-BASU

   Plasmid

   #218449

   Purpose

   expressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integration

   Depositor

   Thorsten Stafforst

   Article

   Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

   Insert

   NLS-Halo-eGFP-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-H2B-AID-EYFP

   Plasmid

   #47329

   Purpose

   for plasmid integration using the Flp-In system. AID at C-terminal

   Depositor

   Don Cleveland

   Article

   Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

   Insert

   histone H2B (H2BC21 Human)
   

   Use

   Tags

   AID and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-FLEXfrt-SaCas9-U6-sgRNA

   Plasmid

   #124845

   Purpose

   Vector for FLP-dependent expression of SaCas9 with gRNA

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SauCas9 gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-fDIO-oG-WPRE-SV40pA

   Plasmid

   #74291

   Purpose

   pAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   oG (optimized Glycoprotein)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   CAG

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAG-FLEx(FRT)-G

   Plasmid

   #67828

   Purpose

   Flp-dependent expression of rabies glycoprotein to mediate trans-synaptic spread of modifed rabies virus in cells

   Depositor

   Liqun Luo

   Article

   Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

   Insert

   rabies glycoprotein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. FR_HNF1A

   Plasmid

   #31104

   Depositor

   Gerhart Ryffel

   Article

   Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

   Insert

   HNF1A (HNF1A Human)
   

   Use

   Flp/frt

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV hSyn-fDIO-taCasp3-TEVp

   Plasmid

   #207997

   Purpose

   Inducing apoptotic ablation of neurons through a process reliant on Flp recombinase

   Depositor

   Kazunari Miyamichi

   Article

   Goto et al Neuron. 2025 Feb 17:S0896-6273(25)00041-

   Insert

   taCasp3-TEVp
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-fDIO-mRuby3-2A-Cre

   Plasmid

   #203844

   Purpose

   Flp-dependent Cre and mRuby3 expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   mRuby3-2A

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE

   Plasmid

   #203842

   Purpose

   Flp-dependent Cre and EGFP expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   EGFP-2A

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

    Plasmid

    #71761

    Purpose

    mGFP and Synaptophysin-mRuby expression from Flp-expressing neurons

    Depositor

    Liqun Luo

    Article

    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

    Inserts

    mGFP
    Synaptophysin
    

    Use

    AAV

    Tags

    Palmitoylation signal and mRuby

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre

    Plasmid

    #203838

    Purpose

    Flp-dependent Cre and mScarlet-KASH expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    mScarlet-KASH-2A

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF5B-FRT-AP-Smo-YFP-DEST

    Plasmid

    #49099

    Purpose

    Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Smo (Smo Mouse)
    

    Use

    Tags

    YFP and acceptor peptide (AP)

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

    Plasmid

    #52889

    Purpose

    "CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

    Depositor

    Iswar Hariharan

    Article

    Bosch et al Development. 2015 Feb 1;142(3):597-606.

    Insert

    Gal4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. CAG-FLEx(FRT)-TC

    Plasmid

    #67827

    Purpose

    Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

    Depositor

    Liqun Luo

    Article

    Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

    Insert

    TVA-mCherry fusion
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5 FRT TO-EGFP-G3BP1-WT

    Plasmid

    #136003

    Purpose

    DOX-inducible WT G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5_FRT_TO_TurboID-mCherry-Cry2

    Plasmid

    #166504

    Purpose

    Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

    Depositor

    Angelos Constantinou

    Article

    Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

    Insert

    None
    

    Use

    Tags

    TurboID and mCherry-CRY2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. c3GIC9

    Plasmid

    #62191

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDragon-empty

    Plasmid

    #155018

    Purpose

    Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-fDIO-mRuby3-2A-dCre

    Plasmid

    #203845

    Purpose

    Flp-dependent enzymatically inactive Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dCre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Expression

    Mutation

    Enzymatically inactive Cre

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDragon-DTA

    Plasmid

    #155017

    Purpose

    For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to attenuated diphtheria toxin (tox176) in the presence of Cre and (r)tTA activity

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Insert

    DTA
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    tox176

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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