Addgene

We narrowed to 5,143 results for: PEP

Showing: 921 - 940 of 5143 results

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pET28a-Cys-ELP(120nm)-LPETGG

Plasmid

#90472

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(120nm)-LPETGG

Use

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8

Plasmid

#169962

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGS_N_3xFLAG

Plasmid

#101654

Purpose

Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, 3xFLAG tag

Use

Tags

3xFLAG tag

Expression

Insect

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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bdSUMO-R9-GFP-HiBit

Plasmid

#170990

Purpose

For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-R9-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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KBB221

Plasmid

#185071

Purpose

SST::ACT2 "SA236"

Depositor

Ken Belanger

Article

Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

Insert

ACT2

Use

Tags

Expression

Yeast

Mutation

ACT2-SA236

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KBB215

Plasmid

#185068

Purpose

SST::WAK2 EcoRI/ScaI fragment (secretion confirmed)

Depositor

Ken Belanger

Article

Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

Insert

WAK2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 ZUC

Plasmid

#169964

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND

Plasmid

#169963

Purpose

pDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 Ub-S297

Plasmid

#169965

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Ub

Expression

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGS_C_3xFLAG

Plasmid

#101653

Purpose

Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, 3xFLAG tag

Use

Tags

3xFLAG tag

Expression

Insect

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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csd5_41-192/pET28b(+)

Plasmid

#83296

Purpose

csd5, construct: 41-192, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1250

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-40

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd4_22-438/pET28b(+)

Plasmid

#83295

Purpose

csd4, construct: 22-438, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1075

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-21

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

Plasmid

#207664

Purpose

Neuropeptide degradation

Depositor

Sung Han

Article

Kim et al bioRxiv 2023.01.19.524797

Insert

NEP (MME Human)

Use

AAV and Cre/Lox

Tags

mRUBY3

Expression

Mutation

Addition of signaling peptide (POMC) on the N-ter…

Promoter

hSynapsin

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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VP16-ER alpha

Plasmid

#11351

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER alpha (ESR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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TPP1-bio-His

Plasmid

#52090

Purpose

Expresses full-length Tripeptidyl-peptidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TPP1 (TPP1 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLEX305-N-dTAG-CARD8

Plasmid

#169989

Purpose

Mammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

dTAG

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-E5TAT-GFP-HiBit

Plasmid

#170993

Purpose

For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-E5TAT-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG

Plasmid

#169991

Purpose

Mammalian purification construct for CARD8

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

TEV-GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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