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We narrowed to 2,469 results for: dcas9

Showing: 921 - 940 of 2469 results

  1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid
    #129032
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA4 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129032-plasmid-map-sequence-id-256156

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  2. IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

    Plasmid
    #121825
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    Sa-dCas9/p300core (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121825-plasmid-map-sequence-id-237377

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  3. IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L

    Plasmid
    #121826
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    Sa-dCas9/Dnmt3a-3L (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121826-plasmid-map-sequence-id-237378

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  4. IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR

    Plasmid
    #121822
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    Sa-dCas9/VPR (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121822-plasmid-map-sequence-id-237380

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  5. IB801: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-KRAB

    Plasmid
    #121823
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    Sa-dCas9/KRAB (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121823-plasmid-map-sequence-id-237375

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  14. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102245-plasmid-map-sequence-id-354473

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  16. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

    Plasmid
    #175571
    Purpose
    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175571-plasmid-map-sequence-id-344994

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  19. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid
    #188899
    Purpose
    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Zim3-dCas9
    Use
    Lentiviral
    Tags
    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion
    Expression
    Mutation
    Promoter
    SFFV
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188899-plasmid-map-sequence-id-374831

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Showing: 921 - 940 of 2469 results