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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,291 results for: grna

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Showing: 9461 - 9480 of 16291 results

  1. pNSU299_CUP1-1

    Plasmid
    #166086
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CUP1-1 (CUP1-1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166086-plasmid-map-sequence-id-330352

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  2. pNSU299_TDH3

    Plasmid
    #166084
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of TDH3 (TDH3 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166084-plasmid-map-sequence-id-329123

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  5. pE4F1.1.0-gDNA

    Plasmid
    #132451
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    E4F1 (E4F1 Human)
    Use
    CRISPR
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    132451-plasmid-map-sequence-id-266819

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  6. alpha4-nAChR shRNA (Alpha-4.3)

    Plasmid
    #86652
    Purpose
    expression of shRNA targeting CHRNA4
    Depositor
    Jaime Modiano
    Insert
    CHRNA4 (alpha4-nAChR) (CHRNA4 Human)
    Use
    RNAi
    Expression
    Mammalian
    Promoter
    H1
    Available Since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    86652-plasmid-map-sequence-id-180128

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  7. psgRNA_EcoWG1

    Pooled Library
    #131625
    Purpose
    Bacterial inhibition library targeting every small RNA and ORF of the E. coli MG1655 genome. This library has higher inhibition efficiency than the psgRNA library.
    Depositor
    David Bikard
    Article
    Calvo-Villamanan et al Nucleic Acids Res. 2020 Apr 30. pii: 582
    Expression
    Bacterial
    Species
    Escherichia coli
    Use
    CRISPR
    Available Since
    May 12, 2020
    Availability
    Academic Institutions and Nonprofits only

  12. psgRNA Library

    Pooled Library
    #115927
    Purpose
    ~93,000 gRNAs targeting random positions along the genome of E. coli MG1655 with a NGG PAM motif. Contains ~19 targets per gene and can be used with SpdCas9 to perform pooled CRISPRi screens.
    Depositor
    David Bikard
    Article
    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:
    Expression
    Bacterial
    Species
    Escherichia coli
    Use
    CRISPR
    Available Since
    June 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  20. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh and U6
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    91854-plasmid-map-sequence-id-176529

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Showing: 9461 - 9480 of 16291 results