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pTH353-SUP45wt

Plasmid

#29371

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

Wild type gene including genomic sequence 1432 bp…

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only

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pTS2381-Tier1-PhCMV-Igk_TM

Plasmid

#169487

Purpose

Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven membrane localization

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-zDicer

Plasmid

#171539

Purpose

Encodes Zebrafish Dicer protein

Depositor

Yan Zeng

Article

Li et al Biochem Biophys Res Commun. 2019 Jun 18;

Insert

dicer1(MK642699) (dicer1 Zebrafish)

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK211

Plasmid

#110595

Purpose

BTK Type 6 Repressor CDS for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

LacI reverse

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pOG2003

Plasmid

#49516

Purpose

TALEN backbone for expression of TALE(deltaRDV)-FokI RR in C. elegans

Depositor

Guangshuo Ou

Article

Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. d

Type

Empty backbone

Tags

FokI-RR, HA, and SV40NLS

Expression

Worm

Promoter

hsp-16.2

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Cquin_596

Plasmid

#176669

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Centrin3

Plasmid

#136807

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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mEos2-Alpha-Actinin-C-14

Plasmid

#57347

Purpose

Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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MTK3_002

Plasmid

#123719

Purpose

Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Blasticydin Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreenF

Plasmid

#78181

Purpose

Expresses rsGreenF in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreenF

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pVH615

Plasmid

#169604

Purpose

Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (A1-pA::PCREm4-Nluc-pA::PmPGK1-Fluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette

Expression

Mammalian

Promoter

PCREm4 / PPGK

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE

Plasmid

#118279

Purpose

Expresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cells

Depositor

Dietmar Schmitz

Article

Wozny et al Front Cell Neurosci. 2018 Oct 2;12:337.

Inserts

mRuby2
ChR2(H134R)

Use

AAV and Cre/Lox

Tags

SV40 NLS and YFP

Promoter

EF1 alpha

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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PcDNA-DEST47-CypB-Del3 C-term GFP

Plasmid

#36136

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 39-216 of cyclophilin B; G63…

Promoter

CMV

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
pMM532

Plasmid

#112529

Purpose

pZA1D-hrtR - HrtR expressed consitutively from promoter ProD with RBS2, p15a origin, AmpR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

HrtR

Expression

Bacterial

Promoter

ProD

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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mEos2-Talin-IBS2-7

Plasmid

#57424

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin IBS2 (Tln1 Mouse)

Tags

mEos2

Expression

Mammalian

Mutation

Talin Integrin Binding Site

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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CROP_C9_Puro_ESR1

Plasmid

#183288

Purpose

All-in-One CRISPRko system with a guide RNA that targets ESR1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ESR1

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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mTFP1-Tubulin-6

Plasmid

#55512

Purpose

Localization: Microtubules, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Tubulin (TUBA1B Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mr in pUASP

Plasmid

#112981

Purpose

P element vector for transposon insertion of mr (APC2) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kashevsky et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

Insert

mr (APC2) (mr Fly)

Use

P insertion vector

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-145

Plasmid

#90505

Purpose

Empty plasmid, pSIN-EF1-alpha-IRES-puro

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Type

Empty backbone

Use

Lentiviral

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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MTK2_016

Plasmid

#123708

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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