We narrowed to 36,026 results for: CaS
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Plasmid#208606PurposeExpressing QuasAr6a_Citrine with an alternate codonDepositorInsertQuasAr6a
UseLentiviralTagsCitrinePromoterhSynAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_591
Plasmid#160653PurposeExpresses OLE1 under the control of the yeast Sup35 promoterDepositorAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(T304I)
Plasmid#177133PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)DepositorAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(K72A)
Plasmid#177132PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)DepositorAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-228)-H6
Plasmid#183779PurposeExpress PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.DepositorAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-390)-H6
Plasmid#183781PurposeExpress PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assaysDepositorInsertPolycomb (w Fly)
Tags6 His tag at the C-terminusExpressionBacterialMutation6 His tag at the C-terminusPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PC(1-86)-H6
Plasmid#183780PurposeExpress PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assaysDepositorAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔ(N+HAT)
Plasmid#182841PurposeAn EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP (w Fly)
TagsFLAG tagExpressionInsectMutationdletion of residues 1566-2142 from CBP1476-3222PromoterMTAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-RG-Linker-eGFP
Plasmid#176071PurposeEGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassetteDepositorInsertRG
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-kappa-myc-dK-2xG4S-TMst
Plasmid#145766PurposeExpresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.DepositorInsertkappa-myc-dK-2xG4S-TMst
ExpressionMammalianMutationThe dK FAP is dL5 with mutations at E50K, L89S, a…PromoterCMVAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro
Plasmid#177145PurposeLentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassetteDepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-PolB(T304I)-Puro
Plasmid#177143PurposeLentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassetteDepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-NQO1(Y128F)
Plasmid#177141PurposeBacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F)DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Nuclease
Plasmid#176528PurposeDelivers all prime editing nuclease components in a single plasmidDepositorInsertCbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
UseCRISPRExpressionMammalianMutationGC to CA point mutation in SpCas9 to restore nucl…PromoterCbH for Cas9, hU6 for gRNAsAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B H85E K88E I92E
Plasmid#175033PurposeExpresses WIPI2B (H85E K88E I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (H85E K88E I92E) (WIPI2 Human)
TagsHaloTagExpressionMammalianMutationHistadine 85 to Glutamate (CAC to GAG); Lysine 88…PromoterCMVAvailable SinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) H158C
Plasmid#134691PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labelingDepositorInsertDouble C2 protein beta (Doc2b Rat)
TagsGSTExpressionBacterialMutationH158C, C145A, C217A, C249A, C290A, C338A, C387AAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) F222C
Plasmid#134692PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labelingDepositorInsertDouble C2 protein beta (Doc2b Rat)
TagsGSTExpressionBacterialMutationF222C, C145A, C217A, C249A, C290A, C338A, C387AAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) A298C
Plasmid#134693PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labelingDepositorInsertDouble C2 protein beta (Doc2b Rat)
TagsGSTExpressionBacterialMutationA298C, C145A, C217A, C249A, C290A, C338A, C387AAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1-K77R
Plasmid#174813PurposeBacterial expression of inactive ALC1 (K77R disrupts ATP binding)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1-E175Q
Plasmid#174814PurposeBacterial expression of inactive ALC1 (E175Q disrupts ATP binding)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only