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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,472 results for: sequence

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Showing: 9501 - 9520 of 13472 results

  3. pEABR NA 3C FLAG SA

    Plasmid
    #234987
    Purpose
    For production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surface
    Depositor
    Monica Gonzalez-Magaldi, Daniel Leahy
    Article
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Insert
    EABR-NA
    Tags
    HRV 3C site, FLAG, Strep-Tactin (SA)
    Expression
    Mammalian
    Mutation
    In the Streptavidin coding sequence Glu44Val/Ser4…
    Available Since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234987-plasmid-map-sequence-id-468750

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  4. pBS CMV NA 3C FLAG SA

    Plasmid
    #234993
    Purpose
    Expression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG protein
    Depositor
    Monica Gonzalez-Magaldi, Daniel Leahy
    Article
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Insert
    NA
    Tags
    HRV 3C site, FLAG, Strep-Tactin (SA)
    Expression
    Mammalian
    Mutation
    In the Streptavidin coding sequence Glu44Val/Ser4…
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234993-plasmid-map-sequence-id-468948

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  7. pLJM1-FIRE-pHLy

    Plasmid
    #170775
    Purpose
    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    CMV
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170775-plasmid-map-sequence-id-339023

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  10. pECNUS4

    Plasmid
    #184887
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184887-plasmid-map-sequence-id-437896

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  12. pAAV.cTNT.iCre

    Plasmid
    #69916
    Purpose
    Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.
    Depositor
    William Pu
    Article
    Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:
    Has Service
    AAV9
    Insert
    improved cre (iCre) recombinase and tdTomato
    Use
    AAV
    Tags
    The iCre coding frame and tdTomato coding frame i…
    Promoter
    Chicken cardiac troponin T
    Available Since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    69916-plasmid-map-sequence-id-472137

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  13. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166698-plasmid-map-sequence-id-329407

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  16. pJBEI-6409

    Plasmid
    #47048
    Purpose
    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Expression
    Bacterial
    Mutation
    R364S mutation in MK
    Promoter
    PlacUV5
    Available Since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47048-plasmid-map-sequence-id-496831

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  17. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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  18. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

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  19. pcDNA3.1-CMV-CFP;UBC-Cre25nt

    Plasmid
    #65727
    Purpose
    Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.
    Depositor
    Jacco van Rheenen
    Article
    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1
    Inserts
    CMV promoter driving CFP expression
    UBC promoter driving Cre-25nt expression
    Use
    Cre/Lox
    Expression
    Mammalian
    Promoter
    CMV and UBC
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    65727-plasmid-map-sequence-id-243566

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  20. mClY-N1

    Plasmid
    #90457
    Purpose
    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.
    Depositor
    Joseph Santos-Sacchi
    Article
    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1
    Insert
    mClY-N1
    Expression
    Mammalian
    Mutation
    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…
    Available Since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90457-plasmid-map-sequence-id-178580

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Showing: 9501 - 9520 of 13472 results