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pSPL3-hRPH3A_c.444Tmut

Plasmid

#190477

Purpose

minigene assay

Depositor

Alfredo Brusco

Article

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Insert

RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)

Expression

Mammalian

Mutation

c.444G>T

Promoter

SV40

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 ORF6 C-V5-tag

Plasmid

#179970

Purpose

Mammalian expression vector for SARS-CoV-1 ORF6, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 ORF6 (ORF6 Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 E C-V5-tag

Plasmid

#179966

Purpose

Mammalian expression vector for SARS-CoV-1 E, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 E (E Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP16 C-V5-tag

Plasmid

#179965

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp16, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 Nsp16 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP-YAP-C-IDR

Plasmid

#166441

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-C-IDR(266-504) (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 rtTA BirA eIF4G +MIC

Plasmid

#158790

Purpose

pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioID

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon)

Tags

3xFlag and BirA*

Expression

Mammalian

Promoter

miniCMV

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Mouse Chromatin Regulator Library

Pooled Library

#200011

Purpose

Knockout library targeting a comprehensive set of mouse genes with annotated function as chromatin regulators.

Depositor

Bradley Bernstein, Gabriel Griffin, Robert Manguso

Article

Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only
Barcode Library 0 (BCL0)

Pooled Library

#179851

Purpose

Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code ACGT.

Depositor

Jason Moffat

Article

Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

Expression

Mammalian

Use

Lentiviral

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only
Barcode Library 1 (BCL1)

Pooled Library

#179852

Purpose

Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code CCAA.

Depositor

Jason Moffat

Article

Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

Expression

Mammalian

Use

Lentiviral

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only
pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

Plasmid

#237480

Purpose

Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.

Depositor

Dirk Daelemans

Article

Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

Insert

SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)

Use

Lentiviral

Tags

IRES-mNeonGreen-NES/PKI and P2A-Blasticidin

Expression

Mammalian

Mutation

S484D + S813I + deleted amino acids 68-76 + 676-6…

Promoter

CMV

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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R620-M18-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 K417T/E484K/N501Y

Plasmid

#170202

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

K417T, E484K, N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-CALR-WT

Plasmid

#194578

Purpose

Mammalian Expression of mEGFP-CALR WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

CALR (CALR Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_BA.2.12.1

Plasmid

#186809

Purpose

pseudovirus plasmid that expresses spike of BA.2.12.1

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2.12.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

L452Q, S704L on the basis of BA.2, c-terminal 18 …

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-CALR-MUT

Plasmid

#194579

Purpose

Mammalian Expression of mEGFP-CALR Mutant (D387Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

CALR (CALR Human)

Tags

mEGFP

Expression

Mammalian

Mutation

D387Rfs*44

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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MG1655 ΔendA ΔrecA

Bacterial Strain

#37853

Depositor

Kristala Prather

Article

Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

Bacterial Resistance

None

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
HL 1255

Bacterial Strain

#52941

Purpose

MG1655 + fimS (off orientation)::gfp::Asp terminator::CamR at fimA

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Bacterial Resistance

Chloramphenicol

Species

Escherichia coli

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
HL 1285

Bacterial Strain

#52942

Purpose

MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Bacterial Resistance

None

Species

Escherichia coli

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
HL 713

Bacterial Strain

#52931

Purpose

MG 1655 + lacIq_KanR inserted into the chromosome at the intS site

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Bacterial Resistance

Kanamycin

Species

Escherichia coli

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
HL3546

Bacterial Strain

#37986

Depositor

Han Lim

Article

Lim et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Bacterial Resistance

Kanamycin

Available Since

July 5, 2012

Availability

Academic Institutions and Nonprofits only
HL 751

Bacterial Strain

#52932

Purpose

MG 1655 + lacIq inserted into the chromosome at the intS site + Δhfq_KanR

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Bacterial Resistance

Kanamycin

Species

Escherichia coli

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only