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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,604 results for: LEA

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Showing: 9521 - 9540 of 16604 results

  1. CPH3736_GST-TEV-Spt6p-tSH2-1223-1454

    Plasmid
    #227088
    Purpose
    Bacterial expression of R1282H/K1355A/K1435A triple-mutant GST-tagged Spt6 tSH2 domain (1223-1454)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H, K1355A, K1435A
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227088-plasmid-map-sequence-id-451126

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  2. CUP1-∆ssCPY*-mScarletI

    Plasmid
    #221157
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221157-plasmid-map-sequence-id-450959

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  3. CUP1-∆ssCPY*-mNeonGreen

    Plasmid
    #221156
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mNeonGreen
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221156-plasmid-map-sequence-id-450957

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  4. pET24a MDH_HALMA

    Plasmid
    #204241
    Purpose
    Bacterial expression of H. marismortui (Dead Sea halophilic red archaeon) cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_HALMA (mdh Haloarcula marismortui)
    Tags
    6X His
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204241-plasmid-map-sequence-id-413344

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  5. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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  6. pT2iC6FAST1nls2ER4(#638)

    Plasmid
    #184068
    Purpose
    cyclofen-inducible YFAST nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    YFAST-2nls-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    184068-plasmid-map-sequence-id-365820

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  7. pFLAG GLTPD2

    Plasmid
    #170745
    Purpose
    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.
    Depositor
    Rhoderick Brown
    Insert
    GLTPD2 (GLTPD2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    V49F, V152A and D209E- Please see depositor comme…
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170745-plasmid-map-sequence-id-337083

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  8. pKP1411

    Plasmid
    #167638
    Purpose
    Express FepA I14C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA I14C
    Expression
    Bacterial
    Mutation
    fepA with I14C mutation (Mutation is I36C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167638-plasmid-map-sequence-id-339563

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  9. pKP1383

    Plasmid
    #167639
    Purpose
    Express FepA V15C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA V15C
    Expression
    Bacterial
    Mutation
    fepA with V15C mutation (Mutation is V37C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167639-plasmid-map-sequence-id-339571

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  10. pKP1384

    Plasmid
    #167641
    Purpose
    Express FepA V16C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA V16C
    Expression
    Bacterial
    Mutation
    fepA with V16C mutation (Mutation is V38C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167641-plasmid-map-sequence-id-339577

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  11. pKP1416

    Plasmid
    #167643
    Purpose
    Express FepA T17C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA T17C
    Expression
    Bacterial
    Mutation
    fepA with T17C mutation (Mutation is T39C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167643-plasmid-map-sequence-id-339580

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  12. pKP1583

    Plasmid
    #167645
    Purpose
    Express FepA D34C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA D34C
    Expression
    Bacterial
    Mutation
    fepA with D34C mutation (Mutation is D56C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167645-plasmid-map-sequence-id-339586

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  13. pKP1382

    Plasmid
    #167647
    Purpose
    Express FepA G54C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA G54C
    Expression
    Bacterial
    Mutation
    fepA with G54C mutation (Mutation is G76C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167647-plasmid-map-sequence-id-339587

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  14. pKP718

    Plasmid
    #167636
    Purpose
    Express FepA T13C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA T13C
    Expression
    Bacterial
    Mutation
    fepA with T13C mutation (Mutation is T35C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167636-plasmid-map-sequence-id-339560

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  15. pKP1410

    Plasmid
    #167634
    Purpose
    Express FepA D12C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA D12C
    Expression
    Bacterial
    Mutation
    FepA with D12C mutation. (Numbering based on star…
    Available Since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167634-plasmid-map-sequence-id-337287

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  18. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid
    #132927
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Insert
    humanized SpCas9
    Use
    CRISPR, Gateway Cloning, and Synthetic Biology
    Available Since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    132927-plasmid-map-sequence-id-314451

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  20. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

    Plasmid
    #119229
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    IL3SP-Vegfd-dNdC (Vegfd Mouse)
    Use
    AAV
    Promoter
    CMV
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119229-plasmid-map-sequence-id-239316

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Showing: 9521 - 9540 of 16604 results