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pICSL30004

Plasmid

#50302

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, YFP variant of GFP (Aequorea victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL80004

Plasmid

#50325

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFUS_A2A

Plasmid

#43849

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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p817-mgammaFcry-mCherry

Plasmid

#23154

Depositor

Zbynek Kozmik

Article

Vopalensky et al Biotechniques. 2010 Mar . 48(3):235-6.

Insert

mouse gammaF crystallin promoter:mCherry (Crygf Mouse)

Use

Multicloning site flanked by i-scei sites

Tags

mCherry

Mutation

mouse gammaF crystallin promoter (-225 - +73) pre…

Available Since

April 13, 2010

Availability

Academic Institutions and Nonprofits only

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CBX5 (3I3C)

Plasmid

#25326

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CBX5_CSD6 (CBX5 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
BII-ChBtW

Plasmid

#133368

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRS303 prp8-101

Plasmid

#111307

Purpose

prp8-101

Depositor

Christine Guthrie

Article

Umen et al Genetics. 1996 Jun;143(2):723-39.

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

prp8-101 (E1960K)

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pUHD-SB-mAID/Bla

Plasmid

#171680

Purpose

TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (blasticidin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Tags

mAID

Expression

Mammalian

Promoter

TRE

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ2-(HA)3

Plasmid

#198178

Purpose

Expression of HA-tagged AP-2 μ2 in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-2 μ2

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 80

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pICH72400

Plasmid

#50338

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, Atug7 (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL80016

Plasmid

#50332

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, beta-glucuronidase (E. coli) with two introns

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-hphMX6

Plasmid

#105161

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-hphMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pNH053

Plasmid

#42159

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mCherry-RT-mCherry

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pRD324

Plasmid

#198045

Purpose

Expression of HMfB D14A K30A E34A in E. coli

Depositor

Remus Dame

Article

Erkelens et al Front Microbiol. 2023 Apr 18;14:1166608.

Insert

HMfB D14A K30A E34A

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pISH-Pou2f2

Plasmid

#84320

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pou2f2 (Pou2f2 Mouse)

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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AlACR1_EYFP_pcDNA3.1

Plasmid

#161022

Purpose

Expresses strongly red-shifted AlACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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T1ACR1_EYFP_pcDNA3.1

Plasmid

#161027

Purpose

Expresses strongly red-shifted T1ACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

T1ACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pICH77911

Plasmid

#50342

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ags (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS1

Plasmid

#20238

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS1

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pLSLGUS

Plasmid

#51517

Purpose

pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

GUS
3kb filler sequence

Use

Plant t-dna plasmid

Tags

NLS and flag

Promoter

virion-sense LIR and 2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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