We narrowed to 110,724 results for: %s

1. px458-AMBRA1 #3

   Plasmid

   #172607

   Purpose

   Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against AMBRA1 (AMBRA1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. px458-AMBRA1 (optimized for N-terminal knock-in)

   Plasmid

   #172608

   Purpose

   Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against AMBRA1 (AMBRA1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-U6-sasgRNACMV- mCherry-WPREpA

   Plasmid

   #217015

   Purpose

   S. aureus sgRNAs backbone along with mCherry expression

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TPI-PTC160

   Plasmid

   #130698

   Purpose

   Expresses TPI-PTC160 NMD reporter (It has a Premature termination codon (PTC) at 160th Amino acid)

   Depositor

   Marvin Tanenbaum

   Article

   Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

   Insert

   TPI-PTC160 (TPI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PTC160

   Promoter

   Pcmv

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTpal-fdc

   Plasmid

   #78288

   Purpose

   Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

   Depositor

   David Nielsen

   Article

   McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

   Inserts

   PAL2 (PAL2 Mustard Weed)
   FDC1 (FDC1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   *see below

   Promoter

   trc

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFPV25.1_rpsM_mCerulean3

   Plasmid

   #124904

   Purpose

   rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

   Depositor

   Markus Covert

   Article

   Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

   Insert

   rpsM promoter and mCerulean3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAW-AlphaLobe

   Plasmid

   #113255

   Purpose

   E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

   Depositor

   Jennifer Doudna

   Article

   Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   S. pyogenes Cas9 alpha-helical lobe
   

   Use

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   residues 56–714; See paper for description

   Promoter

   T7

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TPI-PTC1

   Plasmid

   #130699

   Purpose

   Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid)

   Depositor

   Marvin Tanenbaum

   Article

   Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

   Insert

   TPI-PTC1 (TPI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PTC1

   Promoter

   Pcmv

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-U6-sgRNA_SRD5A1-mcherry

   Plasmid

   #159780

   Purpose

   S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A1 gene

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of sgRNA targeting SRD5A1 gene (SRD5A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-Sga-Cas9

    Plasmid

    #227005

    Purpose

    Mammalian expression of Nuclease active S. gallolyticus Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-Sin-Cas9

    Plasmid

    #227006

    Purpose

    Mammalian expression of Nuclease active S. iniae Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-Spa-Cas9

    Plasmid

    #227007

    Purpose

    Mammalian expression of Nuclease active S. parasanguinis Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-Sub-Cas9

    Plasmid

    #227008

    Purpose

    Mammalian expression of Nuclease active S. uberis Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEMS1277

    Plasmid

    #29140

    Purpose

    Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    CAG promoter
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEMS1306

    Plasmid

    #29148

    Purpose

    Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Type

    Empty backbone

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEMS1302

    Plasmid

    #29147

    Purpose

    Plasmid described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Type

    Empty backbone

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-Cre-NLS

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEMS1307

    Plasmid

    #29149

    Purpose

    Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Type

    Empty backbone

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEMS1157

    Plasmid

    #29072

    Purpose

    Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    CAG promoter
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS1301

    Plasmid

    #29146

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Type

    Empty backbone

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Cre-EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEMS1240

    Plasmid

    #29122

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple11
    

    Use

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge