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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,144 results for: LEA

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Showing: 941 - 960 of 16144 results

  1. TrxA-(A AND C) OR T-SpyTag

    Plasmid
    #246947
    Purpose
    Expresses multifunctional construct for (AND)OR-gated release of ThioredoxinA from materials in response to TEV OR (sortase eSrtA(2A9) AND HRV-3C) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246947-plasmid-map-sequence-id-494406

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  2. Neo-A OR T OR C-SpyTag

    Plasmid
    #246948
    Purpose
    Expresses multifunctional construct for OR OR-gated release of Neoleukin (Neo) from materials in response to sortase eSrtA(2A9) OR TEV OR HRV-3C treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246948-plasmid-map-sequence-id-489555

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  3. mGL-A AND T AND C-SpyTag

    Plasmid
    #246949
    Purpose
    Expresses multifunctional construct for AND AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV AND HRV-3C treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246949-plasmid-map-sequence-id-489547

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  4. EGF-A-SpyTag

    Plasmid
    #246944
    Purpose
    Expresses multifunctional construct for YES-gated release of Epidermal Growth Factor (EGF) from materials in response to sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246944-plasmid-map-sequence-id-489549

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  5. bla-A OR T-SpyTag

    Plasmid
    #246945
    Purpose
    Expresses multifunctional construct for OR-gated release of beta-lactamase (bla) from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246945-plasmid-map-sequence-id-489550

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  6. NanoLuc-A AND T-SpyTag

    Plasmid
    #246946
    Purpose
    Expresses multifunctional construct for AND-gated release of NanoLuciferase (NanoLuc) from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag, SsrA
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246946-plasmid-map-sequence-id-489552

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  11. mCherry-PhoCl AND A-SpyTag

    Plasmid
    #246950
    Purpose
    Expresses multifunctional construct for AND-gated release of mCherry from materials in response to 405 nm light AND sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag, SsrA
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246950-plasmid-map-sequence-id-489551

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  12. pTARGEX-CHL

    Plasmid
    #242447
    Purpose
    Targets high levels of recombinant protein to chloroplast. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    Transit peptide from N. tabacum RbcS
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    242447-plasmid-map-sequence-id-490473

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  13. pTARGEX-APO

    Plasmid
    #242446
    Purpose
    Targets high levels of recombinant protein to apoplast. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    N-terminal fusion with PR1b signal peptide
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    242446-plasmid-map-sequence-id-490468

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  14. pTARGEX-ER

    Plasmid
    #242449
    Purpose
    Targets high levels of recombinant protein for retention in ER. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    ER-retrieval motif (KDEL) and Signal peptide deri…
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242449-plasmid-map-sequence-id-489545

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  15. pOCC240

    Plasmid
    #118895
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and mCherry, clea…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118895-plasmid-map-sequence-id-232366

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  16. pOCC210

    Plasmid
    #118896
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and SNAP tag, cle…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118896-plasmid-map-sequence-id-232311

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  17. pTARGEX-VAC

    Plasmid
    #242450
    Purpose
    Targets high levels of recombinant protein to vacuole. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    C-terminal propeptide (CTPP) derived from N. taba…
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242450-plasmid-map-sequence-id-489544

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  19. pcDNA3-FKBP-ICUE3-NLS

    Plasmid
    #138255
    Purpose
    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Article
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Insert
    FKBP-ICUE3-NLS
    Tags
    FKBP, Nuclear localization signal (NLS), cpVenus[…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    138255-plasmid-map-sequence-id-307462

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Showing: 941 - 960 of 16144 results